Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device

Kai Hong Guo; Pei Hsuan Chen; Chieh Lin; Chien Fu Chen; I. Ren Lee; Yi Chun Yeh

doi:10.1021/acssensors.7b00931

Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device

Kai Hong Guo, Pei Hsuan Chen, Chieh Lin, Chien Fu Chen, I. Ren Lee, Yi Chun Yeh^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.

Original language	English
Pages (from-to)	744-748
Number of pages	5
Journal	ACS Sensors
Volume	3
Issue number	4
DOIs	https://doi.org/10.1021/acssensors.7b00931
Publication status	Published - 2018 Apr 27

Keywords

Cupriavidus metallidurans
cueR
paper device
smartphone-based diagnostic system
whole-cell biosensor

ASJC Scopus subject areas

Bioengineering
Instrumentation
Process Chemistry and Technology
Fluid Flow and Transfer Processes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acssensors.7b00931

Cite this

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title = "Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device",

abstract = "This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.",

keywords = "Cupriavidus metallidurans, cueR, paper device, smartphone-based diagnostic system, whole-cell biosensor",

author = "Guo, {Kai Hong} and Chen, {Pei Hsuan} and Chieh Lin and Chen, {Chien Fu} and Lee, {I. Ren} and Yeh, {Yi Chun}",

note = "Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

year = "2018",

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doi = "10.1021/acssensors.7b00931",

language = "English",

volume = "3",

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AU - Guo, Kai Hong

AU - Chen, Pei Hsuan

AU - Lin, Chieh

AU - Chen, Chien Fu

AU - Lee, I. Ren

AU - Yeh, Yi Chun

PY - 2018/4/27

Y1 - 2018/4/27

N2 - This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.

AB - This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.

KW - Cupriavidus metallidurans

KW - cueR

KW - paper device

KW - smartphone-based diagnostic system

KW - whole-cell biosensor

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Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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