Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device

Kai Hong Guo, Pei Hsuan Chen, Chieh Lin, Chien Fu Chen, I. Ren Lee, Yi Chun Yeh

Research output: Contribution to journalArticle

8 Citations (Scopus)


This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.

Original languageEnglish
Pages (from-to)744-748
Number of pages5
JournalACS Sensors
Issue number4
Publication statusPublished - 2018 Apr 27



  • cueR
  • Cupriavidus metallidurans
  • paper device
  • smartphone-based diagnostic system
  • whole-cell biosensor

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes

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