Determination of corticosterone in mouse plasma by a sweeping technique using micellar electrokinetic chromatography

Chun Hsueh Wu, Mei Chuan Chen, An Kai Su, Pin Yan Shu, Shiu Huey Chou, Cheng Huang Lin

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The separation and on-line concentration of corticosterone in mouse blood was achieved by means of capillary electrophoresis/UV absorbance using sodium dodecyl sulfate (SDS) as a surfactant. The procedure involved the use of an on-line sample concentration method by sweeping-micellar electrokinetic chromatography (sweeping-MEKC). Optimal on-line concentration and separation conditions were determined. The detection limit for this method was 5 ng/ml (S/N=3) and photodiode array detection at 247 nm was used for identification. For the analysis of actual samples, corticosterones from blood samples of a non-stressed and stressed mouse were determined. The results show that only a minor amount of corticosterone was produced by a non-stressed mouse, whereas a significant amount was present in the blood sample from a stressed mouse. The method developed here can be used to examine corticosterone levels as a marker of stress in test animals and may also be used for estimating the effect of stress-release medications.

Original languageEnglish
Pages (from-to)317-325
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume785
Issue number2
DOIs
Publication statusPublished - 2003 Mar 5

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Keywords

  • Corticosterone
  • Sweeping

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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