Determination of corticosterone and 17-hydroxycorticosterone in plasma and urine samples by sweeping techniques using micellar electrokinetic chromatography

Mei Chuan Chen, Shiu Huey Chou, Cheng-Huang Lin

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26 Citations (Scopus)

Abstract

The analysis of corticosterone in mouse blood serum (metabolic-stress experiment) and 17-hydroxycorticosterone in human urine (exercise-stress experiment) samples by means of capillary electrophoresis/UV absorbance in conjunction with online sample concentration techniques is described. The use of normal MEKC had an analyte detection limit of 7μg/ml (S/N=3); whereas when online sample concentration methods, including sweeping-micellar electrokinetic chromatography (Sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were used, the detection limits could be improved to 3 and 5ng/ml, respectively. In the analysis of actual samples from animal metabolic-stress experiments (39 mouse), chronically stressed animals showed a higher level (552±152ng/ml) and acute stressed animals showed an intermediate level (375±105ng/ml). In comparison, normal animals show a lower concentration level of corticosterone (153±109ng/ml). In addition, based on a human exercise-stress experiment (seven volunteers), the acute stressed humans (after exercise, 800m of running) show a higher concentration of 17- hydroxycorticosterone (113±55ng/ml for males; 128±25 for females) and the non-stressed humans (before exercise) show a lower concentration (63±37ng/ml for male; 60±20 for female), respectively.

Original languageEnglish
Pages (from-to)347-353
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume801
Issue number2
DOIs
Publication statusPublished - 2004 Mar 5

Fingerprint

Corticosterone
Chromatography
Animals
Urine
Exercise
Plasmas
Physiological Stress
Limit of Detection
Experiments
Micellar Electrokinetic Capillary Chromatography
Capillary electrophoresis
Capillary Electrophoresis
Running
Cations
Volunteers
Blood
Injections
Serum

Keywords

  • 17-Hydroxycorticosterone
  • Corticosterone

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

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title = "Determination of corticosterone and 17-hydroxycorticosterone in plasma and urine samples by sweeping techniques using micellar electrokinetic chromatography",
abstract = "The analysis of corticosterone in mouse blood serum (metabolic-stress experiment) and 17-hydroxycorticosterone in human urine (exercise-stress experiment) samples by means of capillary electrophoresis/UV absorbance in conjunction with online sample concentration techniques is described. The use of normal MEKC had an analyte detection limit of 7μg/ml (S/N=3); whereas when online sample concentration methods, including sweeping-micellar electrokinetic chromatography (Sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were used, the detection limits could be improved to 3 and 5ng/ml, respectively. In the analysis of actual samples from animal metabolic-stress experiments (39 mouse), chronically stressed animals showed a higher level (552±152ng/ml) and acute stressed animals showed an intermediate level (375±105ng/ml). In comparison, normal animals show a lower concentration level of corticosterone (153±109ng/ml). In addition, based on a human exercise-stress experiment (seven volunteers), the acute stressed humans (after exercise, 800m of running) show a higher concentration of 17- hydroxycorticosterone (113±55ng/ml for males; 128±25 for females) and the non-stressed humans (before exercise) show a lower concentration (63±37ng/ml for male; 60±20 for female), respectively.",
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N2 - The analysis of corticosterone in mouse blood serum (metabolic-stress experiment) and 17-hydroxycorticosterone in human urine (exercise-stress experiment) samples by means of capillary electrophoresis/UV absorbance in conjunction with online sample concentration techniques is described. The use of normal MEKC had an analyte detection limit of 7μg/ml (S/N=3); whereas when online sample concentration methods, including sweeping-micellar electrokinetic chromatography (Sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were used, the detection limits could be improved to 3 and 5ng/ml, respectively. In the analysis of actual samples from animal metabolic-stress experiments (39 mouse), chronically stressed animals showed a higher level (552±152ng/ml) and acute stressed animals showed an intermediate level (375±105ng/ml). In comparison, normal animals show a lower concentration level of corticosterone (153±109ng/ml). In addition, based on a human exercise-stress experiment (seven volunteers), the acute stressed humans (after exercise, 800m of running) show a higher concentration of 17- hydroxycorticosterone (113±55ng/ml for males; 128±25 for females) and the non-stressed humans (before exercise) show a lower concentration (63±37ng/ml for male; 60±20 for female), respectively.

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