Detection of tyrosine and monitoring tyrosinase activity using an enzyme cascade-triggered colorimetric reaction

Huei Yu Chen, Yi Chun Yeh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The aromatic amino acid tyrosine is an essential precursor for the synthesis of catecholamines, including l-DOPA, tyramine, and dopamine. A number of metabolic disorders have been linked to abnormal tyrosine levels in biological fluids. In this study, we developed an enzyme cascade-triggered colorimetric reaction for the detection of tyrosine, based on the formation of yellow pigment (betalamic acid) and red fluorometric betaxanthin. Tyrosinase converts tyrosine to l-DOPA, and DOPA-dioxygenase catalyzes oxidative cleavage of l-DOPA into betalamic acid. Response is linear for tyrosine from 5 to 100 μM, and the detection limit (LOD) is 2.74 μM. The enzyme cascade reaction was applied to monitor tyrosinase activity and tyrosinase inhibition assays. Lastly, the performance of the proposed biosensor proved successful in the analysis of urine samples without the need for pre-treatment. This journal is

Original languageEnglish
Pages (from-to)29745-29750
Number of pages6
JournalRSC Advances
Volume10
Issue number50
DOIs
Publication statusPublished - 2020 Aug 12

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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