Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors

You Ren Hsu; Geng Yen Lee; Jen Inn Chyi; Chung Ke Chang; Chih Cheng Huang; Chen Pin Hsu; Tai Huang Huang; Fan Ren; Yu Lin Wang

doi:10.1149/05006.0239ecst

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors

You Ren Hsu, Geng Yen Lee, Jen Inn Chyi, Chung Ke Chang, Chih Cheng Huang, Chen Pin Hsu, Tai Huang Huang, Fan Ren, Yu Lin Wang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

12 Citations (Scopus)

Abstract

AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect the SARS coronavirus (SARS-CoV) nucleocapsid protein interaction without fluorescent labeling. The detection limit in our system was approximately 0.003 nM of protein sample. Our result showed that this technique was more competitive than isotopelabeling EMSA. We demonstrated AlGaN/GaN was highly potential in constructing a semiconductor-based-sensor binding assay to extract the dissociation constants of nucleic acid-protein interaction.

Original language	English
Title of host publication	Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012
Publisher	Electrochemical Society Inc.
Pages	239-243
Number of pages	5
Edition	6
ISBN (Print)	9781607683544
DOIs	https://doi.org/10.1149/05006.0239ecst
Publication status	Published - 2013
Externally published	Yes
Event	Symposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012 - Honolulu, HI, United States Duration: 2012 Oct 7 → 2012 Oct 12

Publication series

Name	ECS Transactions
Number	6
Volume	50
ISSN (Print)	1938-5862
ISSN (Electronic)	1938-6737

Conference

Conference	Symposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012
Country/Territory	United States
City	Honolulu, HI
Period	2012/10/07 → 2012/10/12

ASJC Scopus subject areas

General Engineering

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10.1149/05006.0239ecst

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Hsu, Y. R., Lee, G. Y., Chyi, J. I., Chang, C. K., Huang, C. C., Hsu, C. P., Huang, T. H., Ren, F., & Wang, Y. L. (2013). Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors. In Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012 (6 ed., pp. 239-243). (ECS Transactions; Vol. 50, No. 6). Electrochemical Society Inc.. https://doi.org/10.1149/05006.0239ecst

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors. / Hsu, You Ren; Lee, Geng Yen; Chyi, Jen Inn et al.
Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012. 6. ed. Electrochemical Society Inc., 2013. p. 239-243 (ECS Transactions; Vol. 50, No. 6).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Hsu, YR, Lee, GY, Chyi, JI, Chang, CK, Huang, CC, Hsu, CP, Huang, TH, Ren, F & Wang, YL 2013, Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors. in Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012. 6 edn, ECS Transactions, no. 6, vol. 50, Electrochemical Society Inc., pp. 239-243, Symposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012, Honolulu, HI, United States, 2012/10/07. https://doi.org/10.1149/05006.0239ecst

Hsu YR, Lee GY, Chyi JI, Chang CK, Huang CC, Hsu CP et al. Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors. In Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012. 6 ed. Electrochemical Society Inc. 2013. p. 239-243. (ECS Transactions; 6). doi: 10.1149/05006.0239ecst

Hsu, You Ren ; Lee, Geng Yen ; Chyi, Jen Inn et al. / Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors. Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012. 6. ed. Electrochemical Society Inc., 2013. pp. 239-243 (ECS Transactions; 6).

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abstract = "AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect the SARS coronavirus (SARS-CoV) nucleocapsid protein interaction without fluorescent labeling. The detection limit in our system was approximately 0.003 nM of protein sample. Our result showed that this technique was more competitive than isotopelabeling EMSA. We demonstrated AlGaN/GaN was highly potential in constructing a semiconductor-based-sensor binding assay to extract the dissociation constants of nucleic acid-protein interaction.",

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AU - Huang, Chih Cheng

AU - Hsu, Chen Pin

AU - Huang, Tai Huang

AU - Ren, Fan

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Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors

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