Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors

You Ren Hsu, Geng Yen Lee, Jen Inn Chyi, Chung Ke Chang, Chih Cheng Huang, Chen Pin Hsu, Tai Huang Huang, Fan Ren, Yu Lin Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect the SARS coronavirus (SARS-CoV) nucleocapsid protein interaction without fluorescent labeling. The detection limit in our system was approximately 0.003 nM of protein sample. Our result showed that this technique was more competitive than isotopelabeling EMSA. We demonstrated AlGaN/GaN was highly potential in constructing a semiconductor-based-sensor binding assay to extract the dissociation constants of nucleic acid-protein interaction.

Original languageEnglish
Title of host publicationLow-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012
Pages239-243
Number of pages5
Edition6
DOIs
Publication statusPublished - 2012
EventSymposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012 - Honolulu, HI, United States
Duration: 2012 Oct 72012 Oct 12

Publication series

NameECS Transactions
Number6
Volume50
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

Other

OtherSymposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012
CountryUnited States
CityHonolulu, HI
Period12/10/712/10/12

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Hsu, Y. R., Lee, G. Y., Chyi, J. I., Chang, C. K., Huang, C. C., Hsu, C. P., Huang, T. H., Ren, F., & Wang, Y. L. (2012). Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors. In Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012 (6 ed., pp. 239-243). (ECS Transactions; Vol. 50, No. 6). https://doi.org/10.1149/05006.0239ecst