Depression by a green tea extract of alcohol-induced oxidative stress and lipogenesis in rat liver

Kuo Hsin Chen; Ping Chia Li; Wei Hsiang Lin; Chiang Ting Chien; Boon Hua Low

doi:10.1271/bbb.110163

Depression by a green tea extract of alcohol-induced oxidative stress and lipogenesis in rat liver

Kuo Hsin Chen
, Ping Chia Li
, Wei Hsiang Lin
, Chiang Ting Chien^*
, Boon Hua Low

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

We determined the effects of a green tea extract with 36% alcohol on the blood alcohol content, oxidative stress, lipogenesis, inflammation and liver function of female Wistar rats. Tea alcohol significantly decreased the O ₂ -, H ₂O ₂ and HOCl amounts via catechins and not caffeine. Thirty days of alcohol gavage improved the level of reactive oxygen species (ROS) in the liver, bile and blood, increased the 4-hydroxynonenal-protein adducts, Kupffer cell infiltration and lipid accumulation in the liver, and elevated the plasma alanine aminotransferase level. A western blot analysis showed reduced expression of the oxidative enzymes (CYP2E1 and NADPH oxidase p47phox protein) and lipogenic enzymes (SREBP-1c and fatty acid synthase) in the alcohol-treated liver. Tea alcohol significantly attenuated these elevated parameters. We conclude that the green tea extract in alcohol efficiently reduced the amounts of O ₂ -, H ₂O ₂ and HOCl primarily due to the catechin content, and not caffeine. The developed tea liquor attenuated alcohol-induced oxidative injury and lipogenesis in the liver by the synergetic action of catechins and caffeine.

Original language	English
Pages (from-to)	1668-1676
Number of pages	9
Journal	Bioscience, Biotechnology and Biochemistry
Volume	75
Issue number	9
DOIs	https://doi.org/10.1271/bbb.110163
Publication status	Published - 2011
Externally published	Yes

Keywords

Alcohol
Caffeine
Catechin
Lipogenesis
Oxidative stress

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.110163

Cite this

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title = "Depression by a green tea extract of alcohol-induced oxidative stress and lipogenesis in rat liver",

abstract = "We determined the effects of a green tea extract with 36\% alcohol on the blood alcohol content, oxidative stress, lipogenesis, inflammation and liver function of female Wistar rats. Tea alcohol significantly decreased the O 2 -, H 2O 2 and HOCl amounts via catechins and not caffeine. Thirty days of alcohol gavage improved the level of reactive oxygen species (ROS) in the liver, bile and blood, increased the 4-hydroxynonenal-protein adducts, Kupffer cell infiltration and lipid accumulation in the liver, and elevated the plasma alanine aminotransferase level. A western blot analysis showed reduced expression of the oxidative enzymes (CYP2E1 and NADPH oxidase p47phox protein) and lipogenic enzymes (SREBP-1c and fatty acid synthase) in the alcohol-treated liver. Tea alcohol significantly attenuated these elevated parameters. We conclude that the green tea extract in alcohol efficiently reduced the amounts of O 2 -, H 2O 2 and HOCl primarily due to the catechin content, and not caffeine. The developed tea liquor attenuated alcohol-induced oxidative injury and lipogenesis in the liver by the synergetic action of catechins and caffeine.",

keywords = "Alcohol, Caffeine, Catechin, Lipogenesis, Oxidative stress",

author = "Chen, \{Kuo Hsin\} and Li, \{Ping Chia\} and Lin, \{Wei Hsiang\} and Chien, \{Chiang Ting\} and Low, \{Boon Hua\}",

note = "Funding Information: We thank Mr. Lin Shu-Me of Clinical Toxicology Laboratory for measuring the blood alcohol concentration. We also thank Miss Tsai Ching-Yi and Chang Huei-Min for preparation of the animal surgery. This work was supported in part by the equipment service of the third core laboratory of Department of Medical Research at NTUH and by grants NTUH98-S1141 and NTUH98-M1216, and partly by the National Science Council of the Republic of China (NSC96-2320-B-002-007 and NSC96-2221-E-002-256-MY3).",

year = "2011",

doi = "10.1271/bbb.110163",

language = "English",

volume = "75",

pages = "1668--1676",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Oxford University Press",

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}

TY - JOUR

T1 - Depression by a green tea extract of alcohol-induced oxidative stress and lipogenesis in rat liver

AU - Chen, Kuo Hsin

AU - Li, Ping Chia

AU - Lin, Wei Hsiang

AU - Chien, Chiang Ting

AU - Low, Boon Hua

N1 - Funding Information: We thank Mr. Lin Shu-Me of Clinical Toxicology Laboratory for measuring the blood alcohol concentration. We also thank Miss Tsai Ching-Yi and Chang Huei-Min for preparation of the animal surgery. This work was supported in part by the equipment service of the third core laboratory of Department of Medical Research at NTUH and by grants NTUH98-S1141 and NTUH98-M1216, and partly by the National Science Council of the Republic of China (NSC96-2320-B-002-007 and NSC96-2221-E-002-256-MY3).

PY - 2011

Y1 - 2011

N2 - We determined the effects of a green tea extract with 36% alcohol on the blood alcohol content, oxidative stress, lipogenesis, inflammation and liver function of female Wistar rats. Tea alcohol significantly decreased the O 2 -, H 2O 2 and HOCl amounts via catechins and not caffeine. Thirty days of alcohol gavage improved the level of reactive oxygen species (ROS) in the liver, bile and blood, increased the 4-hydroxynonenal-protein adducts, Kupffer cell infiltration and lipid accumulation in the liver, and elevated the plasma alanine aminotransferase level. A western blot analysis showed reduced expression of the oxidative enzymes (CYP2E1 and NADPH oxidase p47phox protein) and lipogenic enzymes (SREBP-1c and fatty acid synthase) in the alcohol-treated liver. Tea alcohol significantly attenuated these elevated parameters. We conclude that the green tea extract in alcohol efficiently reduced the amounts of O 2 -, H 2O 2 and HOCl primarily due to the catechin content, and not caffeine. The developed tea liquor attenuated alcohol-induced oxidative injury and lipogenesis in the liver by the synergetic action of catechins and caffeine.

AB - We determined the effects of a green tea extract with 36% alcohol on the blood alcohol content, oxidative stress, lipogenesis, inflammation and liver function of female Wistar rats. Tea alcohol significantly decreased the O 2 -, H 2O 2 and HOCl amounts via catechins and not caffeine. Thirty days of alcohol gavage improved the level of reactive oxygen species (ROS) in the liver, bile and blood, increased the 4-hydroxynonenal-protein adducts, Kupffer cell infiltration and lipid accumulation in the liver, and elevated the plasma alanine aminotransferase level. A western blot analysis showed reduced expression of the oxidative enzymes (CYP2E1 and NADPH oxidase p47phox protein) and lipogenic enzymes (SREBP-1c and fatty acid synthase) in the alcohol-treated liver. Tea alcohol significantly attenuated these elevated parameters. We conclude that the green tea extract in alcohol efficiently reduced the amounts of O 2 -, H 2O 2 and HOCl primarily due to the catechin content, and not caffeine. The developed tea liquor attenuated alcohol-induced oxidative injury and lipogenesis in the liver by the synergetic action of catechins and caffeine.

KW - Alcohol

KW - Caffeine

KW - Catechin

KW - Lipogenesis

KW - Oxidative stress

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UR - https://www.scopus.com/pages/publications/80053167253#tab=citedBy

U2 - 10.1271/bbb.110163

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AN - SCOPUS:80053167253

SN - 0916-8451

VL - 75

SP - 1668

EP - 1676

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 9

ER -

Depression by a green tea extract of alcohol-induced oxidative stress and lipogenesis in rat liver

Abstract

Keywords

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