Depression by a green tea extract of alcohol-induced oxidative stress and lipogenesis in rat liver

Kuo Hsin Chen, Ping Chia Li, Wei Hsiang Lin, Chiang Ting Chien, Boon Hua Low

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We determined the effects of a green tea extract with 36% alcohol on the blood alcohol content, oxidative stress, lipogenesis, inflammation and liver function of female Wistar rats. Tea alcohol significantly decreased the O 2 -, H 2O 2 and HOCl amounts via catechins and not caffeine. Thirty days of alcohol gavage improved the level of reactive oxygen species (ROS) in the liver, bile and blood, increased the 4-hydroxynonenal-protein adducts, Kupffer cell infiltration and lipid accumulation in the liver, and elevated the plasma alanine aminotransferase level. A western blot analysis showed reduced expression of the oxidative enzymes (CYP2E1 and NADPH oxidase p47phox protein) and lipogenic enzymes (SREBP-1c and fatty acid synthase) in the alcohol-treated liver. Tea alcohol significantly attenuated these elevated parameters. We conclude that the green tea extract in alcohol efficiently reduced the amounts of O 2 -, H 2O 2 and HOCl primarily due to the catechin content, and not caffeine. The developed tea liquor attenuated alcohol-induced oxidative injury and lipogenesis in the liver by the synergetic action of catechins and caffeine.

Original languageEnglish
Pages (from-to)1668-1676
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number9
DOIs
Publication statusPublished - 2011 Sep 29

Fingerprint

Lipogenesis
Oxidative stress
Tea
Liver
Rats
Oxidative Stress
Alcohols
Catechin
Caffeine
Blood
Sterol Regulatory Element Binding Protein 1
Cytochrome P-450 CYP2E1
Fatty Acid Synthases
Kupffer Cells
NADPH Oxidase
Enzymes
Alanine Transaminase
Bile
Wistar Rats
Infiltration

Keywords

  • Alcohol
  • Caffeine
  • Catechin
  • Lipogenesis
  • Oxidative stress

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Depression by a green tea extract of alcohol-induced oxidative stress and lipogenesis in rat liver. / Chen, Kuo Hsin; Li, Ping Chia; Lin, Wei Hsiang; Chien, Chiang Ting; Low, Boon Hua.

In: Bioscience, Biotechnology and Biochemistry, Vol. 75, No. 9, 29.09.2011, p. 1668-1676.

Research output: Contribution to journalArticle

Chen, Kuo Hsin ; Li, Ping Chia ; Lin, Wei Hsiang ; Chien, Chiang Ting ; Low, Boon Hua. / Depression by a green tea extract of alcohol-induced oxidative stress and lipogenesis in rat liver. In: Bioscience, Biotechnology and Biochemistry. 2011 ; Vol. 75, No. 9. pp. 1668-1676.
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