Delayed flower senescence of Petunia hybrida plants transformed with antisense broccoli ACC synthase and ACC oxidase genes

Li Chun Huang, U. Long Lai, Shang Fa Yang, Mei Ju Chu, Ching I. Kuo, Mei Fong Tsai, Chih-Wen Sun

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Petunia (Petunia × hybrida Hort. Vilm.-Andr.) plants were transformed by Agrobacterium with antisense BoACS1 (broccoli ACC synthase) and antisense BoACO1 (broccoli ACC oxidase) coding sequences of enzymes involved in biosynthesis of ethylene in broccoli plants. The integration of these genes with an antisense orientation was verified by PCR analyses of kanamycin-resistant regenerants. The expression of transgenes and endogenous genes was further confirmed by RT-PCR analysis. Production of ethylene in shoot tissues was reduced among most transgenic plants. Flowers of transformants, especially excised flowers, generally remained fresh longer than those of untransformed controls. The delayed flower senescence was more pronounced with the antisense BoACO1 than the antisense BoACS1. Transgenic tissues were, nevertheless, still responsive to ethylene. We conclude that the antisense BoACO1 gene from Brassica oleracea is able to reduce ethylene biosynthesis and delay flower senescence of Petunia hybrida more efficiently than the antisense BoACS1 gene.

Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalPostharvest Biology and Technology
Volume46
Issue number1
DOIs
Publication statusPublished - 2007 Oct 1

Fingerprint

1-aminocyclopropanecarboxylate synthase
Petunia
1-aminocyclopropane-1-carboxylate synthase
aminocyclopropanecarboxylate oxidase
Petunia hybrida
Brassica
broccoli
ethylene production
flowers
Genes
genes
Polymerase Chain Reaction
Agrobacterium
Kanamycin
Genetically Modified Plants
kanamycin
Brassica oleracea
Transgenes
transgenes
ethylene

Keywords

  • 1-Aminocyclopropane-1-carboxylic acid (ACC)
  • ACC oxidase (ACO)
  • ACC synthase (ACS)
  • Antisense transgene
  • Ethylene
  • Flower senescence
  • Petunia

ASJC Scopus subject areas

  • Food Science
  • Agronomy and Crop Science
  • Horticulture

Cite this

Delayed flower senescence of Petunia hybrida plants transformed with antisense broccoli ACC synthase and ACC oxidase genes. / Huang, Li Chun; Lai, U. Long; Yang, Shang Fa; Chu, Mei Ju; Kuo, Ching I.; Tsai, Mei Fong; Sun, Chih-Wen.

In: Postharvest Biology and Technology, Vol. 46, No. 1, 01.10.2007, p. 47-53.

Research output: Contribution to journalArticle

Huang, Li Chun ; Lai, U. Long ; Yang, Shang Fa ; Chu, Mei Ju ; Kuo, Ching I. ; Tsai, Mei Fong ; Sun, Chih-Wen. / Delayed flower senescence of Petunia hybrida plants transformed with antisense broccoli ACC synthase and ACC oxidase genes. In: Postharvest Biology and Technology. 2007 ; Vol. 46, No. 1. pp. 47-53.
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