Cysteine-specific blue fluorescence probe

Gondi Sudershan Reddy, Hsuan Ying Chen, I. Jy Chang

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A strong fluorescence probe has been designed to investigate cytochrome c folding kinetics. The cysteine specific modifier, 2-[(-Iodo-acetyl)-methyl- amino]-6-methylaminobenzoic acid methyl ester (1), has been prepared, and the reaction with cysteine can be performed in aqueous solution to ensure its application to protein modification. The model compound, 2-{[2-(2-Acetylamino-2- methyoxycarbonylethylsulfanyl)-acetyl]-methyl-amino}-6-methyl amino-benzoic acid methyl ester (2), resulting from the reaction of 1 with N-acetyl cysteine has emission quantum yields of 0.627 and 0.185 in nonaqueous and aqueous buffer solutions, respectively. The lifetime of 4.6 ns for compound 2 manifests a singlet excited-state. The Förster energy transfer distance of compound 2 and cytochrome c is calculated to be 45 Å.

Original languageEnglish
Pages (from-to)1303-1308
Number of pages6
JournalJournal of the Chinese Chemical Society
Volume53
Issue number6
DOIs
Publication statusPublished - 2006 Jan 1

Keywords

  • Cysteine-specific
  • Energy-transfer
  • Fluorescence
  • Heme protein

ASJC Scopus subject areas

  • Chemistry(all)

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