Cut feather containing rachis as a sampling way for avian sexing

Lih Chiann Wang, Chi Tsong Chen, Hsiao Yuan Lee, Shou-Hsien Li, Jihn Tsair Lir, Shin Chien Chin, Chang En Pu, Ching Ho Wang

Research output: Contribution to journalArticle

4 Citations (Scopus)


Sex determination of birds is important to ensure successful breeding strategies, especially for endangered species. Most birds are vulnerable to stress during handling, however, so obtaining a sufficient amount of genomic DNA (gDNA) while causing the least amount of harm is a critical issue. Avian gender can be determined based on different CHD1 gene intron sizes in W and Z sex chromosomes. We have compared various specimen sources and have found that the rachis segment of a feather is a good DNA source for determining sex. This indicates that plucking the whole feather is not necessary; a cut feather including the rachis is a superior method because it decreases stress on the examined birds and is accomplished easily.

Original languageEnglish
Pages (from-to)279-283
Number of pages5
JournalZoo Biology
Issue number4
Publication statusPublished - 2006 Jul 1


  • CHD1
  • Intron
  • Sex determination
  • Stress

ASJC Scopus subject areas

  • Animal Science and Zoology

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  • Cite this

    Wang, L. C., Chen, C. T., Lee, H. Y., Li, S-H., Lir, J. T., Chin, S. C., Pu, C. E., & Wang, C. H. (2006). Cut feather containing rachis as a sampling way for avian sexing. Zoo Biology, 25(4), 279-283.