Curcumin-induced mitotic spindle defect and cell cycle arrest in human bladder cancer cells occurs partly through inhibition of aurora A

Hsiao Sheng Liu, Ching Shiun Ke, Hung Chi Cheng, Chi Ying F Huang, Chun Li Su

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Curcumin, an active compound in turmeric and curry, has been proven to induce tumor apoptosis and inhibit tumor proliferation, invasion, angiogenesis, and metastasis via modulating numerous targets in various types of cancer cells. Aurora A is a mitosis-related serine-threonine kinase and plays important roles in diverse human cancers. However, the effect of curcumin on Aurora A has not been reported. In this study, Aurora A promoter activity and mRNA expression were inhibited in curcumin-treated human bladder cancer T24 cells, suggesting that Aurora A is regulated at the transcription level. We also found that curcumin preferentially inhibited the growth of T24 cells, which show a higher proliferation rate, invasion activity, and expression level of Aurora A compared with that of human immortalized uroepithelial E7cells. Furthermore, inhibition of phosphorylation of Aurora A and its downstream target histone H3 accompanied by the formation of monopolar spindle, induction of G 2/M phase arrest, and reduction in cell division in response to curcumin were detected in T24 cells. These curcumin-induced phenomena were similar to those using Aurora A small interfering RNA and were attenuated by ectopic expression of Aurora A. Therefore, the antitumor mechanism of curcumin is Aurora A-related, which further supports the application of curcumin in treatments of human cancers.

Original languageEnglish
Pages (from-to)638-646
Number of pages9
JournalMolecular Pharmacology
Volume80
Issue number4
DOIs
Publication statusPublished - 2011 Oct 1

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Spindle Apparatus
Curcumin
Cell Cycle Checkpoints
Urinary Bladder Neoplasms
Neoplasms
Cell Division
Curcuma
Protein-Serine-Threonine Kinases
Mitosis
Histones
Small Interfering RNA
Phosphorylation
Apoptosis
Neoplasm Metastasis
Messenger RNA
Growth

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

Cite this

Curcumin-induced mitotic spindle defect and cell cycle arrest in human bladder cancer cells occurs partly through inhibition of aurora A. / Liu, Hsiao Sheng; Ke, Ching Shiun; Cheng, Hung Chi; Huang, Chi Ying F; Su, Chun Li.

In: Molecular Pharmacology, Vol. 80, No. 4, 01.10.2011, p. 638-646.

Research output: Contribution to journalArticle

Liu, Hsiao Sheng ; Ke, Ching Shiun ; Cheng, Hung Chi ; Huang, Chi Ying F ; Su, Chun Li. / Curcumin-induced mitotic spindle defect and cell cycle arrest in human bladder cancer cells occurs partly through inhibition of aurora A. In: Molecular Pharmacology. 2011 ; Vol. 80, No. 4. pp. 638-646.
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