Cultivation-based characterization of microbial communities associated with deep sedimentary rocks from Taiwan Chelungpu Drilling Project cores

Pei Ling Wang, Li Hung Lin, Hon Tsen Yu, Ting Wen Cheng, Sheng Rong Song, Li Wei Kuo, En Chao Yeh, Weiren Lin, Chien Ying Wang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The Taiwan Chelungpu Drilling Project (TCDP) has provided an unprecedented opportunity to reveal a terrestrial subsurface microbial ecosystem that has possibly experienced continuous disturbance by the arc-continental collision since 5 Ma. The drilling penetrated Pliocene-Pleistocene sedimentary rocks to a depth of 2000 meters below the land surface (mbls) and encountered two major fault zones. Sixteen samples retrieved from the drilled cores at depths between 464 to 1451 mbls were examined to characterize microbial community structures through cultivation-based approaches. Cultivation experiments were performed with various media at temperatures ranging from 30 to 50°C. The results indicate that fermenters and heterotrophic sulfate reducers, using complex organic carbon, were ubiquitously present in most samples. Acetate-utilizing and H2-utilizing sulfate reducers were restricted to shallower intervals along the depth profile. Iron reducers and methanogens were only cultivated in a few shallow samples. Twelve pure strains including 8 fermenters, 3 iron reducers and 1 sulfate reducer originally enriched at 30 or 40°C were isolated and identified with 16S rDNA sequence analyses. They were phylogenetically affiliated with Firmicutes, Bactetiodes, Actinobacteria, and Proteobacteria in various degrees of similarity. The presence of metabolism was not correlated with lithology, depth, temperature and the appearance of fracture throughout the core. The ubiquitous appearance of fermentation and organotrophic sulfate reduction suggests that organic carbon sources were readily accessible in this deep terrestrial subsurface environment. These results infer that the foreland sedimentary strata disturbed by tectonic activities over a geological time scale might support a heterotrophy-dominated deep terrestrial subsurface microbial ecosystem.

Original languageEnglish
Pages (from-to)395-412
Number of pages18
JournalTerrestrial, Atmospheric and Oceanic Sciences
Volume18
Issue number2
DOIs
Publication statusPublished - 2007 Jun 1

Fingerprint

sedimentary rock
microbial community
drilling
sulfate
land surface
organic carbon
heterotrophy
iron
ecosystem
geological time
continental collision
fermentation
fault zone
acetate
Pliocene
lithology
community structure
metabolism
temperature
Pleistocene

Keywords

  • Cultivation
  • Metabolism
  • Microbial community
  • Taiwan Chelungpu Drilling Project

ASJC Scopus subject areas

  • Oceanography
  • Atmospheric Science
  • Earth and Planetary Sciences (miscellaneous)

Cite this

Cultivation-based characterization of microbial communities associated with deep sedimentary rocks from Taiwan Chelungpu Drilling Project cores. / Wang, Pei Ling; Lin, Li Hung; Yu, Hon Tsen; Cheng, Ting Wen; Song, Sheng Rong; Kuo, Li Wei; Yeh, En Chao; Lin, Weiren; Wang, Chien Ying.

In: Terrestrial, Atmospheric and Oceanic Sciences, Vol. 18, No. 2, 01.06.2007, p. 395-412.

Research output: Contribution to journalArticle

Wang, Pei Ling ; Lin, Li Hung ; Yu, Hon Tsen ; Cheng, Ting Wen ; Song, Sheng Rong ; Kuo, Li Wei ; Yeh, En Chao ; Lin, Weiren ; Wang, Chien Ying. / Cultivation-based characterization of microbial communities associated with deep sedimentary rocks from Taiwan Chelungpu Drilling Project cores. In: Terrestrial, Atmospheric and Oceanic Sciences. 2007 ; Vol. 18, No. 2. pp. 395-412.
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AU - Kuo, Li Wei

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