Conversion of crude Jatropha curcas seed oil into biodiesel using liquid recombinant Candida rugosa lipase isozymes

Ting Chun Kuo, Jei Fu Shaw, Guan-Chiun Lee

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31 Citations (Scopus)


The versatile Candida rugosa lipase (CRL) has been widely used in biotechnological applications. However, there have not been feasibility reports on the transesterification of non-edible oils to produce biodiesel using the commercial CRL preparations, mixtures of isozymes. In the present study, four liquid recombinant CRL isozymes (CRL1-CRL4) were investigated to convert various non-edible oils into biodiesel. The results showed that recombinant CRL2 and CRL4 exhibited superior catalytic efficiencies for producing fatty acid methyl ester (FAME) from Jatropha curcas seed oil. A maximum 95.3% FAME yield was achieved using CRL2 under the optimal conditions (50. wt% water, an initial 1. equivalent of methanol feeding, and an additional 0.5. equivalents of methanol feeding at 24. h for a total reaction time of 48. h at 37. °C). We concluded that specific recombinant CRL isozymes could be excellent biocatalysts for the biodiesel production from low-cost crude Jatropha oil.

Original languageEnglish
Pages (from-to)54-59
Number of pages6
JournalBioresource Technology
Publication statusPublished - 2015 Sep 1



  • Biodiesel
  • Candida rugosa lipase (CRL) isozyme
  • Crude seed oil
  • Jatropha curcas
  • Soluble lipase

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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