Conversion of crude Jatropha curcas seed oil into biodiesel using liquid recombinant Candida rugosa lipase isozymes

Ting Chun Kuo, Jei Fu Shaw, Guan-Chiun Lee

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The versatile Candida rugosa lipase (CRL) has been widely used in biotechnological applications. However, there have not been feasibility reports on the transesterification of non-edible oils to produce biodiesel using the commercial CRL preparations, mixtures of isozymes. In the present study, four liquid recombinant CRL isozymes (CRL1-CRL4) were investigated to convert various non-edible oils into biodiesel. The results showed that recombinant CRL2 and CRL4 exhibited superior catalytic efficiencies for producing fatty acid methyl ester (FAME) from Jatropha curcas seed oil. A maximum 95.3% FAME yield was achieved using CRL2 under the optimal conditions (50. wt% water, an initial 1. equivalent of methanol feeding, and an additional 0.5. equivalents of methanol feeding at 24. h for a total reaction time of 48. h at 37. °C). We concluded that specific recombinant CRL isozymes could be excellent biocatalysts for the biodiesel production from low-cost crude Jatropha oil.

Original languageEnglish
Pages (from-to)54-59
Number of pages6
JournalBioresource Technology
Volume192
DOIs
Publication statusPublished - 2015 Sep 1

Fingerprint

Oilseeds
Biofuels
Candida
Lipases
Biodiesel
Lipase
Isoenzymes
seed
ester
liquid
methanol
oil
Liquids
fatty acid
Fatty acids
Methanol
Esters
Oils
Fatty Acids
Biocatalysts

Keywords

  • Biodiesel
  • Candida rugosa lipase (CRL) isozyme
  • Crude seed oil
  • Jatropha curcas
  • Soluble lipase

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Conversion of crude Jatropha curcas seed oil into biodiesel using liquid recombinant Candida rugosa lipase isozymes. / Kuo, Ting Chun; Shaw, Jei Fu; Lee, Guan-Chiun.

In: Bioresource Technology, Vol. 192, 01.09.2015, p. 54-59.

Research output: Contribution to journalArticle

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