Contribution of creatine to protein homeostasis in athletes after endurance and sprint running

Fu Chun Tang, Chun Chen Chan, Po Ling Kuo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Purpose: Few studies have focused on the metabolic changes induced by creatine supplementation. This study investigated the effects of creatine supplementation on plasma and urinary metabolite changes of athletes after endurance and sprint running. Methods: Twelve male athletes (20.3 ± 1.4 y) performed two identical (65-70 % maximum heart rate reserved) 60 min running exercises (endurance trial) before and after creatine supplementation (12 g creatine monohydrate/day for 15 days), followed by a 5-day washout period. Subsequently, they performed two identical 100 m sprint running exercises (power trial) before and after 15 days of creatine supplementation in accordance with the supplementary protocol of the endurance trial. Body composition measurements were performed during the entire study. Plasma samples were examined for the concentrations of glucose, lactate, branched-chain amino acids (BCAAs), free-tryptophan (f-TRP), glutamine, alanine, hypoxanthine, and uric acid. Urinary samples were examined for the concentrations of hydroxyproline, 3-methylhistidine, urea nitrogen, and creatinine. Results: Creatine supplementation significantly increased body weights of the athletes of endurance trial. Plasma lactate concentration and ratio of f-TRP/BCAAs after recovery from endurance running were significantly decreased with creatine supplementation. Plasma purine metabolites (the sum of hypoxanthine and uric acid), glutamine, urinary 3-methylhistidine, and urea nitrogen concentrations tended to decrease before running in trials with creatine supplements. After running, urinary hydroxyproline concentration significantly increased in the power trial with creatine supplements. Conclusions: The findings suggest that creatine supplementation tended to decrease muscle glycogen and protein degradation, especially after endurance exercise. However, creatine supplementation might induce collagen proteolysis in athletes after sprint running.

Original languageEnglish
Pages (from-to)61-71
Number of pages11
JournalEuropean Journal of Nutrition
Volume53
Issue number1
DOIs
Publication statusPublished - 2014 Feb 1

Fingerprint

Creatine
Running
Athletes
Homeostasis
Proteins
Branched Chain Amino Acids
Hypoxanthine
Hydroxyproline
Exercise
Uric Acid
Glutamine
Tryptophan
Proteolysis
Urea
Lactic Acid
Nitrogen
Muscle Proteins
Clinical Protocols
Body Composition
Glycogen

Keywords

  • 3-Methylhistidine
  • Alanine
  • Glutamine
  • Hydroxyproline
  • Purine metabolites
  • Urinary urea nitrogen

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Contribution of creatine to protein homeostasis in athletes after endurance and sprint running. / Tang, Fu Chun; Chan, Chun Chen; Kuo, Po Ling.

In: European Journal of Nutrition, Vol. 53, No. 1, 01.02.2014, p. 61-71.

Research output: Contribution to journalArticle

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abstract = "Purpose: Few studies have focused on the metabolic changes induced by creatine supplementation. This study investigated the effects of creatine supplementation on plasma and urinary metabolite changes of athletes after endurance and sprint running. Methods: Twelve male athletes (20.3 ± 1.4 y) performed two identical (65-70 {\%} maximum heart rate reserved) 60 min running exercises (endurance trial) before and after creatine supplementation (12 g creatine monohydrate/day for 15 days), followed by a 5-day washout period. Subsequently, they performed two identical 100 m sprint running exercises (power trial) before and after 15 days of creatine supplementation in accordance with the supplementary protocol of the endurance trial. Body composition measurements were performed during the entire study. Plasma samples were examined for the concentrations of glucose, lactate, branched-chain amino acids (BCAAs), free-tryptophan (f-TRP), glutamine, alanine, hypoxanthine, and uric acid. Urinary samples were examined for the concentrations of hydroxyproline, 3-methylhistidine, urea nitrogen, and creatinine. Results: Creatine supplementation significantly increased body weights of the athletes of endurance trial. Plasma lactate concentration and ratio of f-TRP/BCAAs after recovery from endurance running were significantly decreased with creatine supplementation. Plasma purine metabolites (the sum of hypoxanthine and uric acid), glutamine, urinary 3-methylhistidine, and urea nitrogen concentrations tended to decrease before running in trials with creatine supplements. After running, urinary hydroxyproline concentration significantly increased in the power trial with creatine supplements. Conclusions: The findings suggest that creatine supplementation tended to decrease muscle glycogen and protein degradation, especially after endurance exercise. However, creatine supplementation might induce collagen proteolysis in athletes after sprint running.",
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