Confocal mapping of myelin figures with micro-Raman spectroscopy

Jung Ren Huang; Yu Che Cheng; Hung Ji Huang; Hai Pang Chiang

doi:10.1007/s00339-017-1450-z

Confocal mapping of myelin figures with micro-Raman spectroscopy

Jung Ren Huang^*, Yu Che Cheng, Hung Ji Huang, Hai Pang Chiang

^*Corresponding author for this work

Department of Physics

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We employ confocal micro-Raman spectroscopy (CMRS) with submicron spatial resolution to study the myelin structures (cylindrical lamellae) composed of nested surfactant C₁₂E₃ or lipid DMPC bilayers. The CMRS mapping indicates that for a straight C₁₂E₃ myelin, the surfactant concentration increases with the myelin width and is higher in the center region than in the peripheral region. For a curved C₁₂E₃ myelin, the convex side has a higher surfactant concentration than the corresponding concave side. The spectrum of DMPC myelins undergoes a qualitative change as the temperature increases above 60 °C, suggesting that the surfactant molecules may be damaged. Our work demonstrates the utility of CMRS in bio-soft material research.

Original language	English
Article number	58
Journal	Applied Physics A: Materials Science and Processing
Volume	124
Issue number	1
DOIs	https://doi.org/10.1007/s00339-017-1450-z
Publication status	Published - 2018 Jan 1

ASJC Scopus subject areas

General Chemistry
General Materials Science

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abstract = "We employ confocal micro-Raman spectroscopy (CMRS) with submicron spatial resolution to study the myelin structures (cylindrical lamellae) composed of nested surfactant C12E3 or lipid DMPC bilayers. The CMRS mapping indicates that for a straight C12E3 myelin, the surfactant concentration increases with the myelin width and is higher in the center region than in the peripheral region. For a curved C12E3 myelin, the convex side has a higher surfactant concentration than the corresponding concave side. The spectrum of DMPC myelins undergoes a qualitative change as the temperature increases above 60 °C, suggesting that the surfactant molecules may be damaged. Our work demonstrates the utility of CMRS in bio-soft material research.",

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AU - Cheng, Yu Che

AU - Huang, Hung Ji

AU - Chiang, Hai Pang

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N2 - We employ confocal micro-Raman spectroscopy (CMRS) with submicron spatial resolution to study the myelin structures (cylindrical lamellae) composed of nested surfactant C12E3 or lipid DMPC bilayers. The CMRS mapping indicates that for a straight C12E3 myelin, the surfactant concentration increases with the myelin width and is higher in the center region than in the peripheral region. For a curved C12E3 myelin, the convex side has a higher surfactant concentration than the corresponding concave side. The spectrum of DMPC myelins undergoes a qualitative change as the temperature increases above 60 °C, suggesting that the surfactant molecules may be damaged. Our work demonstrates the utility of CMRS in bio-soft material research.

AB - We employ confocal micro-Raman spectroscopy (CMRS) with submicron spatial resolution to study the myelin structures (cylindrical lamellae) composed of nested surfactant C12E3 or lipid DMPC bilayers. The CMRS mapping indicates that for a straight C12E3 myelin, the surfactant concentration increases with the myelin width and is higher in the center region than in the peripheral region. For a curved C12E3 myelin, the convex side has a higher surfactant concentration than the corresponding concave side. The spectrum of DMPC myelins undergoes a qualitative change as the temperature increases above 60 °C, suggesting that the surfactant molecules may be damaged. Our work demonstrates the utility of CMRS in bio-soft material research.

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Confocal mapping of myelin figures with micro-Raman spectroscopy

Abstract

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