Comparison of the use of anionic and cationic surfactants for the separation of steroids based on MEKC and sweeping-MEKC modes

Hui Ju Shen, Cheng Huang Lin

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

In attempts to improve the selectivity and sensitivity of steroid separation and to determine their migration order, comparison of the use of anionic and cationic surfactants based on the MEKC and sweeping-MEKC modes was made. A mixture of six steroids (progesterone, 17-hydroxy progesterone, 11-deoxycortisol, corticosterone, cortisone, and cortisol) could be separated and detected by means of the CE/UV-absorption method. The order of migration time for these steroids was compared under various conditions, including acidic/alkaline buffers, anionic/cationic surfactants, and positive/negative applied voltage, causing the direction of the EOF and the migration of micelles to change. The major rules for generally predicting the migration order of steroids are summarized. The detection limits were significantly improved when the sweeping-MEKC mode was applied.

Original languageEnglish
Pages (from-to)1255-1262
Number of pages8
JournalElectrophoresis
Volume27
Issue number5-6
DOIs
Publication statusPublished - 2006 Mar 1

Keywords

  • Anionic and cationic surfactants
  • MEKC
  • Progesterone
  • Steroids
  • Sweeping MEKC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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