Comparison of the separation of nine tryptamine standards based on gas chromatography, high performance liquid chromatography and capillary electrophoresis methods

Man Juing Wang, Ju Tsung Liu, Hung Ming Chen, Jian Jhih Lin, Cheng Huang Lin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Nine tryptamines, including α-methyltryptamine (AMT), N,N-dimethyltryptamine (DMT), 5-methoxy-α-methyltryptamine (5-MeO-AMT), N,N-diethyltryptamine (DET), N,N-dipropyltryptamine (DPT), N,N-dibutyltryptamine (DBT), N,N-diisopropyltryptamine (DIPT), 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT), and 5-methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) were selected as model compounds. Comparisons of their sensitivity, selectivity, time, cost and the order of migration are described based on different separation techniques (GC, HPLC and CE, respectively). As a result, the limit of detection (S/N = 3) obtained by GC/MS and LC/UV-absorption ranged from 0.5 to 15 μg/mL and 0.3 to 1.0 μg/mL, respectively. In contrast to this, based on the CZE/UV-absorption method, the limit of detection (S/N = 3) was determined to 0.5-1 μg/mL. However, when the sweeping-MEKC mode was applied, it dramatically improved to 2-10 ng/mL. In the case of GC, HPLC and CE, migration times of the nine standards ranged from 11 to 15 min and 8 to 23 min by GC and HPLC, respectively; ranged from 20 to 26 min by sweeping-MEKC. The order of migration of DMT, DET, DPT and DBT follows the molecular weight, whereas the order of migration of AMT and 5-MeO-AMT (primary amines), DIPT (an isomer of DPT) and 5-methoxy-tryptamines (5-MeO-AMT, 5-MeO-DMT and 5-MeO-DIPT) can be altered by changing the separation conditions.

Original languageEnglish
Pages (from-to)131-136
Number of pages6
JournalJournal of Chromatography A
Volume1181
Issue number1-2
DOIs
Publication statusPublished - 2008 Feb 15

Fingerprint

Methoxydimethyltryptamines
Capillary electrophoresis
High performance liquid chromatography
Gas chromatography
Tryptamines
N,N-Dimethyltryptamine
Isomers
Amines
Molecular weight
tryptamine
dipropyltryptamine
Costs
5-methoxy-N,N-diisopropyltryptamine

Keywords

  • CE
  • GC
  • HPLC
  • Tryptamine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Comparison of the separation of nine tryptamine standards based on gas chromatography, high performance liquid chromatography and capillary electrophoresis methods. / Wang, Man Juing; Liu, Ju Tsung; Chen, Hung Ming; Lin, Jian Jhih; Lin, Cheng Huang.

In: Journal of Chromatography A, Vol. 1181, No. 1-2, 15.02.2008, p. 131-136.

Research output: Contribution to journalArticle

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abstract = "Nine tryptamines, including α-methyltryptamine (AMT), N,N-dimethyltryptamine (DMT), 5-methoxy-α-methyltryptamine (5-MeO-AMT), N,N-diethyltryptamine (DET), N,N-dipropyltryptamine (DPT), N,N-dibutyltryptamine (DBT), N,N-diisopropyltryptamine (DIPT), 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT), and 5-methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) were selected as model compounds. Comparisons of their sensitivity, selectivity, time, cost and the order of migration are described based on different separation techniques (GC, HPLC and CE, respectively). As a result, the limit of detection (S/N = 3) obtained by GC/MS and LC/UV-absorption ranged from 0.5 to 15 μg/mL and 0.3 to 1.0 μg/mL, respectively. In contrast to this, based on the CZE/UV-absorption method, the limit of detection (S/N = 3) was determined to 0.5-1 μg/mL. However, when the sweeping-MEKC mode was applied, it dramatically improved to 2-10 ng/mL. In the case of GC, HPLC and CE, migration times of the nine standards ranged from 11 to 15 min and 8 to 23 min by GC and HPLC, respectively; ranged from 20 to 26 min by sweeping-MEKC. The order of migration of DMT, DET, DPT and DBT follows the molecular weight, whereas the order of migration of AMT and 5-MeO-AMT (primary amines), DIPT (an isomer of DPT) and 5-methoxy-tryptamines (5-MeO-AMT, 5-MeO-DMT and 5-MeO-DIPT) can be altered by changing the separation conditions.",
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AU - Lin, Cheng Huang

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AB - Nine tryptamines, including α-methyltryptamine (AMT), N,N-dimethyltryptamine (DMT), 5-methoxy-α-methyltryptamine (5-MeO-AMT), N,N-diethyltryptamine (DET), N,N-dipropyltryptamine (DPT), N,N-dibutyltryptamine (DBT), N,N-diisopropyltryptamine (DIPT), 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT), and 5-methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) were selected as model compounds. Comparisons of their sensitivity, selectivity, time, cost and the order of migration are described based on different separation techniques (GC, HPLC and CE, respectively). As a result, the limit of detection (S/N = 3) obtained by GC/MS and LC/UV-absorption ranged from 0.5 to 15 μg/mL and 0.3 to 1.0 μg/mL, respectively. In contrast to this, based on the CZE/UV-absorption method, the limit of detection (S/N = 3) was determined to 0.5-1 μg/mL. However, when the sweeping-MEKC mode was applied, it dramatically improved to 2-10 ng/mL. In the case of GC, HPLC and CE, migration times of the nine standards ranged from 11 to 15 min and 8 to 23 min by GC and HPLC, respectively; ranged from 20 to 26 min by sweeping-MEKC. The order of migration of DMT, DET, DPT and DBT follows the molecular weight, whereas the order of migration of AMT and 5-MeO-AMT (primary amines), DIPT (an isomer of DPT) and 5-methoxy-tryptamines (5-MeO-AMT, 5-MeO-DMT and 5-MeO-DIPT) can be altered by changing the separation conditions.

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