Comparative proteomic profiling of plasma very-low-density and low-density lipoproteins

Hung Yu Sun, Sun Fang Chen, Ming Derg Lai, Ting Tsung Chang, Tz Li Chen, Pei Yu Li, Dar Bin Shieh, Kung Chia Young

Research output: Contribution to journalArticle

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Abstract

Background: Low-density lipoprotein (LDL) is a natural metabolite of very-low-density lipoprotein (VLDL) in the circulation. Systematic investigation of total protein components and dynamics might provide insights into this normal metabolic process. Methods: VLDL and LDL were purified from normolipidemia pooled plasma by gradient ultracentrifugation with either ionic or non-ionic media. The protein contents were compared by liquid chromatography tandem mass analyses based on isobaric tag for relative and absolute quantitation and two-dimensional gel electrophoresis. Results: Our comparative lipoproteomes revealed 21 associated proteins. Combined with Western blot analysis, and on the basis of the differential expression levels we classified them into 3 groups: (i) VLDL > LDL [apolipoprotein (apo) A-IV, apo(a), apoCs, apoE, apoJ and serum amyloid A-4]; (ii) VLDL < LDL [albumin, α-1-antitrypsin, apoD, apoF, apoM, and paraoxonase-1]; and (iii) VLDL = LDL [apoA-I, apoA-II, apoB-100, apoL-I and prenylcysteine oxidase-1]. The apoA-I level positively correlated with PCYOX1 but negatively with apoM in VLDL and LDL. Furthermore, the two-dimensional maps displayed 5 apoA-I isoforms in which phosphorylation at Ser55, Ser166, Thr185, Thr221 and Ser252 residues were identified. Conclusions: This study revealed the VLDL- and LDL lipoproteomes and the full-spectrum protein changes during physiological VLDL-to-LDL transition. It provides a valuable dataset VLDL and LDL proteomes potentially applied to the development of diagnostics.

Original languageEnglish
Pages (from-to)336-344
Number of pages9
JournalClinica Chimica Acta
Volume411
Issue number5-6
DOIs
Publication statusPublished - 2010 Mar 2

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VLDL Lipoproteins
LDL Lipoproteins
Proteomics
Plasmas
Apolipoprotein A-I
Proteins
Apolipoprotein A-II
Apolipoprotein B-100
Apoprotein(a)
Aryldialkylphosphatase
Serum Amyloid A Protein
Phosphorylation
Ultracentrifugation
Liquid chromatography
Electrophoresis, Gel, Two-Dimensional
Apolipoproteins E
Proteome
Metabolites
Electrophoresis
Liquid Chromatography

Keywords

  • Lipoproteome
  • Low-density lipoprotein
  • Quantitative proteomics
  • Two-dimensional electrophoresis
  • Very-low-density lipoprotein
  • iTRAQ

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Sun, H. Y., Chen, S. F., Lai, M. D., Chang, T. T., Chen, T. L., Li, P. Y., ... Young, K. C. (2010). Comparative proteomic profiling of plasma very-low-density and low-density lipoproteins. Clinica Chimica Acta, 411(5-6), 336-344. https://doi.org/10.1016/j.cca.2009.11.023

Comparative proteomic profiling of plasma very-low-density and low-density lipoproteins. / Sun, Hung Yu; Chen, Sun Fang; Lai, Ming Derg; Chang, Ting Tsung; Chen, Tz Li; Li, Pei Yu; Shieh, Dar Bin; Young, Kung Chia.

In: Clinica Chimica Acta, Vol. 411, No. 5-6, 02.03.2010, p. 336-344.

Research output: Contribution to journalArticle

Sun, HY, Chen, SF, Lai, MD, Chang, TT, Chen, TL, Li, PY, Shieh, DB & Young, KC 2010, 'Comparative proteomic profiling of plasma very-low-density and low-density lipoproteins', Clinica Chimica Acta, vol. 411, no. 5-6, pp. 336-344. https://doi.org/10.1016/j.cca.2009.11.023
Sun, Hung Yu ; Chen, Sun Fang ; Lai, Ming Derg ; Chang, Ting Tsung ; Chen, Tz Li ; Li, Pei Yu ; Shieh, Dar Bin ; Young, Kung Chia. / Comparative proteomic profiling of plasma very-low-density and low-density lipoproteins. In: Clinica Chimica Acta. 2010 ; Vol. 411, No. 5-6. pp. 336-344.
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AU - Li, Pei Yu

AU - Shieh, Dar Bin

AU - Young, Kung Chia

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AB - Background: Low-density lipoprotein (LDL) is a natural metabolite of very-low-density lipoprotein (VLDL) in the circulation. Systematic investigation of total protein components and dynamics might provide insights into this normal metabolic process. Methods: VLDL and LDL were purified from normolipidemia pooled plasma by gradient ultracentrifugation with either ionic or non-ionic media. The protein contents were compared by liquid chromatography tandem mass analyses based on isobaric tag for relative and absolute quantitation and two-dimensional gel electrophoresis. Results: Our comparative lipoproteomes revealed 21 associated proteins. Combined with Western blot analysis, and on the basis of the differential expression levels we classified them into 3 groups: (i) VLDL > LDL [apolipoprotein (apo) A-IV, apo(a), apoCs, apoE, apoJ and serum amyloid A-4]; (ii) VLDL < LDL [albumin, α-1-antitrypsin, apoD, apoF, apoM, and paraoxonase-1]; and (iii) VLDL = LDL [apoA-I, apoA-II, apoB-100, apoL-I and prenylcysteine oxidase-1]. The apoA-I level positively correlated with PCYOX1 but negatively with apoM in VLDL and LDL. Furthermore, the two-dimensional maps displayed 5 apoA-I isoforms in which phosphorylation at Ser55, Ser166, Thr185, Thr221 and Ser252 residues were identified. Conclusions: This study revealed the VLDL- and LDL lipoproteomes and the full-spectrum protein changes during physiological VLDL-to-LDL transition. It provides a valuable dataset VLDL and LDL proteomes potentially applied to the development of diagnostics.

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KW - iTRAQ

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