Comparative proteomic profiling of plasma very-low-density and low-density lipoproteins

Hung Yu Sun, Sun Fang Chen, Ming Derg Lai, Ting Tsung Chang, Tz Li Chen, Pei Yu Li, Dar Bin Shieh, Kung Chia Young

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

Background: Low-density lipoprotein (LDL) is a natural metabolite of very-low-density lipoprotein (VLDL) in the circulation. Systematic investigation of total protein components and dynamics might provide insights into this normal metabolic process. Methods: VLDL and LDL were purified from normolipidemia pooled plasma by gradient ultracentrifugation with either ionic or non-ionic media. The protein contents were compared by liquid chromatography tandem mass analyses based on isobaric tag for relative and absolute quantitation and two-dimensional gel electrophoresis. Results: Our comparative lipoproteomes revealed 21 associated proteins. Combined with Western blot analysis, and on the basis of the differential expression levels we classified them into 3 groups: (i) VLDL > LDL [apolipoprotein (apo) A-IV, apo(a), apoCs, apoE, apoJ and serum amyloid A-4]; (ii) VLDL < LDL [albumin, α-1-antitrypsin, apoD, apoF, apoM, and paraoxonase-1]; and (iii) VLDL = LDL [apoA-I, apoA-II, apoB-100, apoL-I and prenylcysteine oxidase-1]. The apoA-I level positively correlated with PCYOX1 but negatively with apoM in VLDL and LDL. Furthermore, the two-dimensional maps displayed 5 apoA-I isoforms in which phosphorylation at Ser55, Ser166, Thr185, Thr221 and Ser252 residues were identified. Conclusions: This study revealed the VLDL- and LDL lipoproteomes and the full-spectrum protein changes during physiological VLDL-to-LDL transition. It provides a valuable dataset VLDL and LDL proteomes potentially applied to the development of diagnostics.

Original languageEnglish
Pages (from-to)336-344
Number of pages9
JournalClinica Chimica Acta
Volume411
Issue number5-6
DOIs
Publication statusPublished - 2010 Mar 2

Keywords

  • Lipoproteome
  • Low-density lipoprotein
  • Quantitative proteomics
  • Two-dimensional electrophoresis
  • Very-low-density lipoprotein
  • iTRAQ

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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