TY - JOUR
T1 - Comparative Expression Profiles of mRNAs and microRNAs Among Human Mesenchymal Stem Cells Derived From Breast, Face, and Abdominal Adipose Tissues
AU - Wang, Kai Hung
AU - Kao, An Pei
AU - Singh, Sher
AU - Yu, Sung Liang
AU - Kao, Li Pin
AU - Yun Tsai, Zong
AU - Lin, Sin Daw
AU - Li, Steven Shoei Lung
N1 - Funding Information:
We thank Dr Tsai-Ming Lin for providing human breast and face adipose tissues. We also appreciate the technical assistance by the research assistants at the Microarray Core Facility directed by Professor Pan-Chyr Yang of the National Research Program for Genomic Medicine of National Science Council in Taiwan. This investigation was supported in part by a National Genomic Medicine grant of the National Science Council in Taiwan (NSC95/96/97-3112-B-037-002), KMU Center of Excellence for Environmental Medicine Project (kmu-em-97-1.3c) to S.S.-L. Li, and the National Science Council, Taiwan (NSC 96-2314-B-037-002) to S.-D. Lin.
PY - 2010/3
Y1 - 2010/3
N2 - We determined the expression of both mRNAs and microRNAs (miRNAs) from human mesenchymal stem cells BM19, FM30, and AM3, which is derived from breast, face, and abdominal adipose tissues, respectively. BM19, FM30, and AM3 cells exhibited considerably similar mRNA profiles, and their 1,038 abundantly common genes were involved in regulating six cell adhesion and three cytoskeleton remodeling processes among the top ten GeneGo canonical pathway maps. The 39 most abundant miRNAs in AM3 cells were expressed at very similar levels in BM19 cells. However, seven abundant miRNAs (miR-19b, miR-320, miR-186, miR-199a, miR-339, miR-99a, and miR-152) in AM3 cells were expressed at much lower levels than that in FM30 cells, and 38 genes targeted by these miRNAs were consequently upregulated more than 3-fold in FM30 cells compared with AM3 cells. Therefore, autologous abdominal adipose-derived mesenchymal stem cells are suitable for tissue engineering of breast reconstruction because of very similar expression profiles of mRNAs and miRNAs between AM3 and BM19 cells. Conversely, abdominal AM3 cells might not be suitable for facial rejuvenation, since the 38 highly expressed genes targeted by miRNAs in FM30 cells might play an important role(s) in the development of facial tissue.
AB - We determined the expression of both mRNAs and microRNAs (miRNAs) from human mesenchymal stem cells BM19, FM30, and AM3, which is derived from breast, face, and abdominal adipose tissues, respectively. BM19, FM30, and AM3 cells exhibited considerably similar mRNA profiles, and their 1,038 abundantly common genes were involved in regulating six cell adhesion and three cytoskeleton remodeling processes among the top ten GeneGo canonical pathway maps. The 39 most abundant miRNAs in AM3 cells were expressed at very similar levels in BM19 cells. However, seven abundant miRNAs (miR-19b, miR-320, miR-186, miR-199a, miR-339, miR-99a, and miR-152) in AM3 cells were expressed at much lower levels than that in FM30 cells, and 38 genes targeted by these miRNAs were consequently upregulated more than 3-fold in FM30 cells compared with AM3 cells. Therefore, autologous abdominal adipose-derived mesenchymal stem cells are suitable for tissue engineering of breast reconstruction because of very similar expression profiles of mRNAs and miRNAs between AM3 and BM19 cells. Conversely, abdominal AM3 cells might not be suitable for facial rejuvenation, since the 38 highly expressed genes targeted by miRNAs in FM30 cells might play an important role(s) in the development of facial tissue.
KW - adipose
KW - genes
KW - mesenchymal stem cells
KW - microRNAs
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U2 - 10.1016/S1607-551X(10)70017-6
DO - 10.1016/S1607-551X(10)70017-6
M3 - Article
C2 - 20227650
AN - SCOPUS:77749273586
SN - 1607-551X
VL - 26
SP - 113
EP - 122
JO - Kaohsiung Journal of Medical Sciences
JF - Kaohsiung Journal of Medical Sciences
IS - 3
ER -