Comparative effects of flavonoids on oxidant scavenging and ischemia-reperfusion injury in cardiomyocytes

Wei Tien Chang, Zuo Hui Shao, Jun Jie Yin, Sangeeta Mehendale, Chong Zhi Wang, Yimin Qin, Juan Li, Wen Jone Chen, Chiang-Ting Chien, Lance B. Becker, Terry L. Vanden Hoek, Chun Su Yuan

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Since flavonoids scavenge reactive oxygen species, they may potentially protect against ischemia/reperfusion injury. This study compared the scavenging capacity of specific flavonoids towards different reactive oxygen species. Whether the differential oxidant scavenging capacity correlated with their protective efficacy in ischemia/reperfusion injury of cardiomyocytes was determined. The free radical scavenging capacity of five flavonoids (wogonin, baicalin, baicalein, catechin and procyanidin B2) was analyzed using electron spin resonance spectrometry for 3 radicals: 1,1-diphenyl-2picrylhydrazyl (DPPH), superoxide and hydroxyl radical. A well-established chick cardiomyocyte model of ischemia (1 h)/reperfusion (3 h) was used to evaluate flavonoid-induced protection against ischemia/reperfusion injury in chronic treatment (pretreated 72 h and treated through ischemia/reperfusion) and acute treatment protocols (during ischemia/reperfusion or only at reperfusion). The cell viability was assessed by propidium iodide. The DPPH scavenging was most significant with catechin, followed by procyanidin B2, baicalein, baicalin, and wogonin. The superoxide scavenging was, similarly, most significant with catechin, followed by baicalein, procyanidin B2, and baicalin. For hydroxyl radical, only baicalein showed a significant scavenging capacity (> 50% reduction in ESR signal). For the cardiomyocyte studies, all flavonoids but wogonin showed protection against ischemia/reperfusion injury in the chronic treatment protocol. When flavonoids were administered only during ischemia/reperfusion, baicalein, procyanidin B2, and catechin significantly reduced cell death. If flavonoids were administered just at reperfusion, only baicalein and procyanidin B2 had protective effects, and the efficacy was less. Flavonoids possess specific but differential radical scavenging capacity, which, in conjunction with the timing of treatment, affects their protective efficacy in cardiomyocytes exposed to ischemia/reperfusion.

Original languageEnglish
Pages (from-to)58-66
Number of pages9
JournalEuropean Journal of Pharmacology
Volume566
Issue number1-3
DOIs
Publication statusPublished - 2007 Jul 2

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Reperfusion Injury
Flavonoids
Cardiac Myocytes
Oxidants
Reperfusion
Catechin
Ischemia
Clinical Protocols
Superoxides
Hydroxyl Radical
Reactive Oxygen Species
Propidium
Electron Spin Resonance Spectroscopy
Free Radicals
baicalein
Spectrum Analysis
Cell Survival
Cell Death
procyanidin B2
Therapeutics

Keywords

  • Flavonoids
  • Hydroxyl radical
  • Reperfusion injury
  • Scavenging capacity
  • Superoxide

ASJC Scopus subject areas

  • Pharmacology

Cite this

Chang, W. T., Shao, Z. H., Yin, J. J., Mehendale, S., Wang, C. Z., Qin, Y., ... Yuan, C. S. (2007). Comparative effects of flavonoids on oxidant scavenging and ischemia-reperfusion injury in cardiomyocytes. European Journal of Pharmacology, 566(1-3), 58-66. https://doi.org/10.1016/j.ejphar.2007.03.037

Comparative effects of flavonoids on oxidant scavenging and ischemia-reperfusion injury in cardiomyocytes. / Chang, Wei Tien; Shao, Zuo Hui; Yin, Jun Jie; Mehendale, Sangeeta; Wang, Chong Zhi; Qin, Yimin; Li, Juan; Chen, Wen Jone; Chien, Chiang-Ting; Becker, Lance B.; Vanden Hoek, Terry L.; Yuan, Chun Su.

In: European Journal of Pharmacology, Vol. 566, No. 1-3, 02.07.2007, p. 58-66.

Research output: Contribution to journalArticle

Chang, WT, Shao, ZH, Yin, JJ, Mehendale, S, Wang, CZ, Qin, Y, Li, J, Chen, WJ, Chien, C-T, Becker, LB, Vanden Hoek, TL & Yuan, CS 2007, 'Comparative effects of flavonoids on oxidant scavenging and ischemia-reperfusion injury in cardiomyocytes', European Journal of Pharmacology, vol. 566, no. 1-3, pp. 58-66. https://doi.org/10.1016/j.ejphar.2007.03.037
Chang, Wei Tien ; Shao, Zuo Hui ; Yin, Jun Jie ; Mehendale, Sangeeta ; Wang, Chong Zhi ; Qin, Yimin ; Li, Juan ; Chen, Wen Jone ; Chien, Chiang-Ting ; Becker, Lance B. ; Vanden Hoek, Terry L. ; Yuan, Chun Su. / Comparative effects of flavonoids on oxidant scavenging and ischemia-reperfusion injury in cardiomyocytes. In: European Journal of Pharmacology. 2007 ; Vol. 566, No. 1-3. pp. 58-66.
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