Colorimetric and Fluorometric Paper-Based Assay for Cu2+ Detection Based on Green Synthesis of 2-Aminoterephthalic Acid-Derived Pigments

Chia I. Shih; Yi Chieh Chou; Huei Yu Chen; Kuan Han Chen; I. Hsiang Wang; Yi Chun Yeh

doi:10.1021/acsabm.0c00212

Colorimetric and Fluorometric Paper-Based Assay for Cu²⁺ Detection Based on Green Synthesis of 2-Aminoterephthalic Acid-Derived Pigments

Chia I. Shih
, Yi Chieh Chou
, Huei Yu Chen
, Kuan Han Chen
, I. Hsiang Wang
, Yi Chun Yeh^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

In this study, we developed a simple and economical method for the green synthesis of Cu²⁺ sensors based on betaxanthin pigments. Aminoisophthalic acid-betaxanthin was synthesized by coupling 2-aminoisophthalic acid and betalamic acid produced from DOPA-extradiol-4,5-dioxygenase in situ and in vitro. The resulting 2-aminoterephthalic acid-betaxanthin (2-AIPA-BX) presented a satisfying fluorescence quantum yield in water and a high degree of selectivity for Cu²⁺ over interfering metal ions. The bioproduction process of 2-AIPA-BX was scaled up from test tubes to 1 L-flasks, indicating the robustness and reproducibility of this method. Additionally, we successfully incorporated 2-AIPA-BX into paper-based analytical devices to facilitate simple, inexpensive, and portable setup with lower sample consumption for onsite monitoring of environmental and biological samples.

Original language	English
Pages (from-to)	2516-2521
Number of pages	6
Journal	ACS Applied Bio Materials
Volume	3
Issue number	4
DOIs	https://doi.org/10.1021/acsabm.0c00212
Publication status	Published - 2020 Apr 20

Keywords

Cu
DOPA 4,5-dioxygenase
betaxanthin
fluorogenic sensors
green synthesis

ASJC Scopus subject areas

Biomaterials
General Chemistry
Biomedical Engineering
Biochemistry, medical

Access to Document

10.1021/acsabm.0c00212

Cite this

@article{a087eddf829340768237d57862e36fb4,

title = "Colorimetric and Fluorometric Paper-Based Assay for Cu2+ Detection Based on Green Synthesis of 2-Aminoterephthalic Acid-Derived Pigments",

abstract = "In this study, we developed a simple and economical method for the green synthesis of Cu2+ sensors based on betaxanthin pigments. Aminoisophthalic acid-betaxanthin was synthesized by coupling 2-aminoisophthalic acid and betalamic acid produced from DOPA-extradiol-4,5-dioxygenase in situ and in vitro. The resulting 2-aminoterephthalic acid-betaxanthin (2-AIPA-BX) presented a satisfying fluorescence quantum yield in water and a high degree of selectivity for Cu2+ over interfering metal ions. The bioproduction process of 2-AIPA-BX was scaled up from test tubes to 1 L-flasks, indicating the robustness and reproducibility of this method. Additionally, we successfully incorporated 2-AIPA-BX into paper-based analytical devices to facilitate simple, inexpensive, and portable setup with lower sample consumption for onsite monitoring of environmental and biological samples.",

keywords = "Cu, DOPA 4,5-dioxygenase, betaxanthin, fluorogenic sensors, green synthesis",

author = "Shih, \{Chia I.\} and Chou, \{Yi Chieh\} and Chen, \{Huei Yu\} and Chen, \{Kuan Han\} and Wang, \{I. Hsiang\} and Yeh, \{Yi Chun\}",

note = "Publisher Copyright: Copyright {\textcopyright} 2020 American Chemical Society.",

year = "2020",

month = apr,

day = "20",

doi = "10.1021/acsabm.0c00212",

language = "English",

volume = "3",

pages = "2516--2521",

journal = "ACS Applied Bio Materials",

issn = "2576-6422",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Colorimetric and Fluorometric Paper-Based Assay for Cu2+ Detection Based on Green Synthesis of 2-Aminoterephthalic Acid-Derived Pigments

AU - Shih, Chia I.

AU - Chou, Yi Chieh

AU - Chen, Huei Yu

AU - Chen, Kuan Han

AU - Wang, I. Hsiang

AU - Yeh, Yi Chun

PY - 2020/4/20

Y1 - 2020/4/20

N2 - In this study, we developed a simple and economical method for the green synthesis of Cu2+ sensors based on betaxanthin pigments. Aminoisophthalic acid-betaxanthin was synthesized by coupling 2-aminoisophthalic acid and betalamic acid produced from DOPA-extradiol-4,5-dioxygenase in situ and in vitro. The resulting 2-aminoterephthalic acid-betaxanthin (2-AIPA-BX) presented a satisfying fluorescence quantum yield in water and a high degree of selectivity for Cu2+ over interfering metal ions. The bioproduction process of 2-AIPA-BX was scaled up from test tubes to 1 L-flasks, indicating the robustness and reproducibility of this method. Additionally, we successfully incorporated 2-AIPA-BX into paper-based analytical devices to facilitate simple, inexpensive, and portable setup with lower sample consumption for onsite monitoring of environmental and biological samples.

AB - In this study, we developed a simple and economical method for the green synthesis of Cu2+ sensors based on betaxanthin pigments. Aminoisophthalic acid-betaxanthin was synthesized by coupling 2-aminoisophthalic acid and betalamic acid produced from DOPA-extradiol-4,5-dioxygenase in situ and in vitro. The resulting 2-aminoterephthalic acid-betaxanthin (2-AIPA-BX) presented a satisfying fluorescence quantum yield in water and a high degree of selectivity for Cu2+ over interfering metal ions. The bioproduction process of 2-AIPA-BX was scaled up from test tubes to 1 L-flasks, indicating the robustness and reproducibility of this method. Additionally, we successfully incorporated 2-AIPA-BX into paper-based analytical devices to facilitate simple, inexpensive, and portable setup with lower sample consumption for onsite monitoring of environmental and biological samples.

KW - Cu

KW - DOPA 4,5-dioxygenase

KW - betaxanthin

KW - fluorogenic sensors

KW - green synthesis

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