Abstract
In this study, we developed a simple and economical method for the green synthesis of Cu2+ sensors based on betaxanthin pigments. Aminoisophthalic acid-betaxanthin was synthesized by coupling 2-aminoisophthalic acid and betalamic acid produced from DOPA-extradiol-4,5-dioxygenase in situ and in vitro. The resulting 2-aminoterephthalic acid-betaxanthin (2-AIPA-BX) presented a satisfying fluorescence quantum yield in water and a high degree of selectivity for Cu2+ over interfering metal ions. The bioproduction process of 2-AIPA-BX was scaled up from test tubes to 1 L-flasks, indicating the robustness and reproducibility of this method. Additionally, we successfully incorporated 2-AIPA-BX into paper-based analytical devices to facilitate simple, inexpensive, and portable setup with lower sample consumption for onsite monitoring of environmental and biological samples.
Original language | English |
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Pages (from-to) | 2516-2521 |
Number of pages | 6 |
Journal | ACS Applied Bio Materials |
Volume | 3 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2020 Apr 20 |
Keywords
- Cu
- DOPA 4,5-dioxygenase
- betaxanthin
- fluorogenic sensors
- green synthesis
ASJC Scopus subject areas
- Biomaterials
- General Chemistry
- Biomedical Engineering
- Biochemistry, medical