Colorimetric and Fluorometric Paper-Based Assay for Cu2+ Detection Based on Green Synthesis of 2-Aminoterephthalic Acid-Derived Pigments

Chia I. Shih, Yi Chieh Chou, Huei Yu Chen, Kuan Han Chen, I. Hsiang Wang, Yi Chun Yeh

Research output: Contribution to journalArticle


In this study, we developed a simple and economical method for the green synthesis of Cu2+ sensors based on betaxanthin pigments. Aminoisophthalic acid-betaxanthin was synthesized by coupling 2-aminoisophthalic acid and betalamic acid produced from DOPA-extradiol-4,5-dioxygenase in situ and in vitro. The resulting 2-aminoterephthalic acid-betaxanthin (2-AIPA-BX) presented a satisfying fluorescence quantum yield in water and a high degree of selectivity for Cu2+ over interfering metal ions. The bioproduction process of 2-AIPA-BX was scaled up from test tubes to 1 L-flasks, indicating the robustness and reproducibility of this method. Additionally, we successfully incorporated 2-AIPA-BX into paper-based analytical devices to facilitate simple, inexpensive, and portable setup with lower sample consumption for onsite monitoring of environmental and biological samples.

Original languageEnglish
Pages (from-to)2516-2521
Number of pages6
JournalACS Applied Bio Materials
Issue number4
Publication statusPublished - 2020 Apr 20


  • betaxanthin
  • Cu
  • DOPA 4,5-dioxygenase
  • fluorogenic sensors
  • green synthesis

ASJC Scopus subject areas

  • Biomaterials
  • Chemistry(all)
  • Biomedical Engineering
  • Biochemistry, medical

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