Colloid-gold nanoparticle enhanced detection sensitivity of paired surface plasma waves biosensor

Hsieh Ting Wu, Yi Ling Chen, Yen Chen Huang, Wei Chih Liu, Ming Yaw Ng, Yin Fong Chang, Bao Yu Hsieh, Chien Chou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Previous work with amplitude-sensitive paired surface plasma waves biosensor (PSPWB) demonstrated that the detection sensitivity of PSPWB is able to experimentally detect 0.001% sucrose-water solution and biomolecular interaction of 10pg/ml mouse IgG interacting with immobilized anti-mouse IgG successfully. Further development of the detection sensitivity of PSPWB has been conducted by using 20nm in diameter colloid gold nanoparticles conjugated with target molecules that can result in a higher mass coverage and a larger resonant angle change of plasmon resonance, thereby its detection sensitivity is further enhanced significantly. Bare gold nanoparticles, which is randomly suspended in solution, is adopted to differentiate biospecific binding induced further signal enhancement Experimentally, the sensitivity at 330fg/ml of Au-nanoparticle conjugated protein A (PA-Au) interacting with mouse IgG which is immobilized on a CM5 sensor chip was detected successfully. By this arrangement, 6-fold signal amplification is demonstrated compared with the same concentration of PA without conjugated gold nanoparticles.

Original languageEnglish
Title of host publicationNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
DOIs
Publication statusPublished - 2007 Apr 30
EventNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV - San Jose, CA, United States
Duration: 2007 Jan 232007 Jan 24

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6447
ISSN (Print)1605-7422

Other

OtherNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
CountryUnited States
CitySan Jose, CA
Period07/1/2307/1/24

Keywords

  • Biosensor
  • Nanoparticles
  • Optical heterodyne
  • Surface plasmon

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Wu, H. T., Chen, Y. L., Huang, Y. C., Liu, W. C., Ng, M. Y., Chang, Y. F., Hsieh, B. Y., & Chou, C. (2007). Colloid-gold nanoparticle enhanced detection sensitivity of paired surface plasma waves biosensor. In Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV [64470S] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6447). https://doi.org/10.1117/12.700009