CO2-driven seawater acidification differentially affects development and molecular plasticity along life history of fish (Oryzias latipes)

Yung Che Tseng, Marian Y. Hu, Meike Stumpp, Li Yih Lin, Frank Melzner, Pung Pung Hwang

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Fish early life stages have been shown to react sensitive to simulated ocean acidification. In particular, acid-base disturbances elicited by altered seawater carbonate chemistry have been shown to induce pathologies in larval fish. However, the mechanisms underlying these disturbances are largely unknown. We used gene expression profiling of genes involved in acid-base regulation and metabolism to investigate the effects of seawater hypercapnia on developing Japanese ricefish (medaka; Oryzias latipes). Our results demonstrate that embryos respond with delayed development during the time window of 2-5dpf when exposed to a seawater pCO2 of 0.12 and 0.42kPa. This developmental delay is associated with strong down-regulation of genes from major metabolic pathways including glycolysis (G6PDH), Krebs cycle (CS) and the electron transport chain (CytC). In a second step we identified acid-base relevant genes in different ontogenetic stages (embryos, hatchlings and adults) and tissues (gill and intestine) that are up regulated in response to hypercapnia, including NHE3, NBCa, NBCb, AE1a, AE1b, ATP1a1a.1, ATP1a1b, ATP1b1a, Rhag, Rhbg and Rhcg. Interestingly, NHE3 and Rhcg expressions were increased in response to environmental hypercapnia in all ontogenetic stages and tissues tested, indicating the central role of these proteins in acid-base regulation. Furthermore, the increased expression of genes from amino acid metabolism pathways (ALT1, ALT2, AST1a, AST1b, AST2 and GLUD) suggests that energetic demands of hatchlings are fueled by the breakdown of amino acids. The present study provides a first detailed gene expression analysis throughout the ontogeny of a euryhaline teleost in response to seawater hypercapnia, indicating highest sensitivity in early embryonic stages, when functional ion regulatory epithelia are not yet developed.

Original languageEnglish
Pages (from-to)119-1130
Number of pages1012
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume165
Issue number2
DOIs
Publication statusPublished - 2013 Jun

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Keywords

  • Acid-base regulation
  • Embryonic development
  • Epithelium
  • Ocean acidification

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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