Co-immobilization of polymeric luminol, iron(II) tris(5-aminophenanthroline) and glucose oxidase at an electrode surface, and its application as a glucose optrode

Ching Hao Wang, Shen Ming Chen, Chong Mou Wang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The anodic polymerization of 3-aminophthalhydrazide (luminol) and iron(II) tris 5-aminophenanthroline (Fe(phen-NH2)32+) has been reported in this paper. A bilayer electrode was developed based on these polymers and the ITO conductive glass (denoted ITO|Fe(phen-NH2)32+|luminol electrode). This electrode emitted light (λem: 430 nm) as it was brought into contact with H2O2. At pH 10, the resulting electrochemiluminescence (ECL) showed a linear relationship with the concentration of H2O2 in the range of 10 μM-1 mM. This bilayer electrode also showed an application potential for the detection of glucose after being further modified with glucose oxidase (denoted ITO|Fe(phen-NH2)32+|luminol|GOx electrode). Although the resulting ECL decayed more rapidly in concentrated glucose solutions (e.g., 1 M) because of the consumption of luminol during use, the decay became less severe in diluted glucose solutions (e.g., 10 mM). According to the flow injection analysis, a linear relationship existed between the ECL and the concentration of glucose from 10-5-10-3 M at pH 9. The detection limit could reach a level of 5 × 10-5 M at this pH.

Original languageEnglish
Pages (from-to)1507-1511
Number of pages5
JournalAnalyst
Volume127
Issue number11
DOIs
Publication statusPublished - 2002 Nov 1

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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