Co-immobilization of polymeric luminol, iron(II) tris(5-aminophenanthroline) and glucose oxidase at an electrode surface, and its application as a glucose optrode

Ching Hao Wang, Shen Ming Chen, Chong Mou Wang

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The anodic polymerization of 3-aminophthalhydrazide (luminol) and iron(II) tris 5-aminophenanthroline (Fe(phen-NH2)32+) has been reported in this paper. A bilayer electrode was developed based on these polymers and the ITO conductive glass (denoted ITO|Fe(phen-NH2)32+|luminol electrode). This electrode emitted light (λem: 430 nm) as it was brought into contact with H2O2. At pH 10, the resulting electrochemiluminescence (ECL) showed a linear relationship with the concentration of H2O2 in the range of 10 μM-1 mM. This bilayer electrode also showed an application potential for the detection of glucose after being further modified with glucose oxidase (denoted ITO|Fe(phen-NH2)32+|luminol|GOx electrode). Although the resulting ECL decayed more rapidly in concentrated glucose solutions (e.g., 1 M) because of the consumption of luminol during use, the decay became less severe in diluted glucose solutions (e.g., 10 mM). According to the flow injection analysis, a linear relationship existed between the ECL and the concentration of glucose from 10-5-10-3 M at pH 9. The detection limit could reach a level of 5 × 10-5 M at this pH.

Original languageEnglish
Pages (from-to)1507-1511
Number of pages5
JournalAnalyst
Volume127
Issue number11
DOIs
Publication statusPublished - 2002 Nov 1

Fingerprint

Luminol
Glucose Oxidase
Glucose oxidase
Immobilization
immobilization
Glucose
Electrodes
electrode
glucose
Iron
iron
Flow Injection Analysis
ITO glass
polymerization
Polymerization
Glass
Limit of Detection
Polymers
polymer
glass

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Co-immobilization of polymeric luminol, iron(II) tris(5-aminophenanthroline) and glucose oxidase at an electrode surface, and its application as a glucose optrode. / Wang, Ching Hao; Chen, Shen Ming; Wang, Chong Mou.

In: Analyst, Vol. 127, No. 11, 01.11.2002, p. 1507-1511.

Research output: Contribution to journalArticle

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