Chinese Herbal Medicine Glycyrrhiza inflata Reduces Aβ Aggregation and Exerts Neuroprotection through Anti-Oxidation and Anti-Inflammation

Ya Jen Chiu, Chi Mei Lee, Te Hsien Lin, Hsuan Yuan Lin, Shin Ying Lee, Mina Mesri, Kuo Hsuan Chang, Jung Yaw Lin, Guey Jen Lee-Chen, Chiung Mei Chen

Research output: Contribution to journalArticle

Abstract

Amyloid β (Aβ) plays a major role in the pathogenesis of Alzheimer's disease (AD). The accumulation of misfolded Aβ causes oxidative and inflammatory damage leading to apoptotic cell death. Chinese herbal medicine (CHM) has been widely used in clinical practice to treat neurodegenerative diseases associated with oxidative stress and neuroinflammation. This study examined the neuroprotection effects of CHM extract Glycyrrhiza inflata (G. inflata) and its active constituents, licochalcone A and liquiritigenin in AD. We examined Aβ aggregation inhibition, anti-oxidation and neuroprotection in Tet-On Aβ-GFP 293/SH-SY5Y cells and anti-inflammatory potential in lipopolysaccharide (LPS)-stimulated RAW 264.7 and LPS and interferon (IFN)-γ (LPS/IFN-γ)-activated BV-2 cells. In addition, we applied conditioned media (CM) of BV-2 cells primed with LPS/IFN-γ to Aβ-GFP SH-SY5Y cells to uncover the neuroprotective mechanisms. Our results showed that G. inflata extract and its two constituents displayed potentials of Aβ aggregation inhibition and radical-scavenging in biochemical assays, Aβ misfolding inhibition and reactive oxygen species (ROS) reduction in Aβ-GFP 293 cells, as well as neurite outgrowth promotion, acetylcholinesterase inhibition and SOD2 up-regulation in Aβ-GFP SH-SY5Y cells. Meanwhile, both G. inflata extract and its constituents suppressed NO, TNF-α, IL-1β, PGE2 and/or Iba1 productions in inflammation-stimulated RAW 264.7 or BV-2 cells. G. inflata extract and its constituents further protected Aβ-GFP SH-SY5Y cells from BV-2 CM-induced cell death by ameliorating reduced BCL2 and attenuating increased IGFBP2, cleaved CASP3, BAD and BAX. Collectively, G. inflata extract, licochalcone A and liquiritigenin display neuroprotection through exerting anti-oxidative and anti-inflammatory activities to suppress neuronal apoptosis.

Original languageEnglish
Pages (from-to)1535-1549
Number of pages15
JournalAmerican Journal of Chinese Medicine
Volume46
Issue number7
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

Glycyrrhiza
Herbal Medicine
Amyloid
Apoptosis
Inflammation
Lipopolysaccharides
Conditioned Culture Medium
Interferon-gamma
Alzheimer Disease
Cell Death
Anti-Inflammatory Agents
Neuroprotection
Acetylcholinesterase
Dinoprostone
Caspase 3
Neurodegenerative Diseases
Reactive Oxygen Species
Oxidative Stress
Up-Regulation
Tumor Necrosis Factor-alpha

Keywords

  • Amyloid β
  • Anti-Inflammation
  • Anti-Oxidation
  • Apoptosis
  • G. inflata Extract
  • Liquiritigenin/Licochalcone A
  • Neuroprotection

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Chinese Herbal Medicine Glycyrrhiza inflata Reduces Aβ Aggregation and Exerts Neuroprotection through Anti-Oxidation and Anti-Inflammation. / Chiu, Ya Jen; Lee, Chi Mei; Lin, Te Hsien; Lin, Hsuan Yuan; Lee, Shin Ying; Mesri, Mina; Chang, Kuo Hsuan; Lin, Jung Yaw; Lee-Chen, Guey Jen; Chen, Chiung Mei.

In: American Journal of Chinese Medicine, Vol. 46, No. 7, 01.01.2018, p. 1535-1549.

Research output: Contribution to journalArticle

Chiu, Ya Jen ; Lee, Chi Mei ; Lin, Te Hsien ; Lin, Hsuan Yuan ; Lee, Shin Ying ; Mesri, Mina ; Chang, Kuo Hsuan ; Lin, Jung Yaw ; Lee-Chen, Guey Jen ; Chen, Chiung Mei. / Chinese Herbal Medicine Glycyrrhiza inflata Reduces Aβ Aggregation and Exerts Neuroprotection through Anti-Oxidation and Anti-Inflammation. In: American Journal of Chinese Medicine. 2018 ; Vol. 46, No. 7. pp. 1535-1549.
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