Characterizations of anti-alpha-fetoprotein- conjugated magnetic nanoparticles associated with alpha-fetoprotein for biomedical applications

Shu Hsien Liao, Han Sheng Huang, Jen Jie Chieh, Yu Kai Su, Yuan Fu Tong, Kai Wen Huang

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    In this work, we report characterizations of biofunctionalized magnetic nanoparticles (BMNPs) associated with alpha-fetoprotein (AFP) for biomedical applications. The example BMNP in this study is anti-alpha-fetoprotein (anti-AFP) conjugated onto dextran-coated Fe3O4 labeled as Fe3O4-anti-AFP, and the target is AFP.We characterize magnetic properties, such as increments of magnetization ΔMH and effective relaxation time Δτeff in the reaction process. It is found that both ΔMH and Δτeff are enhanced when the concentration of AFP, ΦAFP, increases. The enhancements are due to magnetic interactions among BMNPs in magnetic clusters, which contribute extra MH after the association with MH and in turn enhance τeff. The screening of patients carrying hepatocellular carcinoma (HCC) is verified via ΔMH/MH. The proposed method can be applied to detect a wide variety of analytes. The scaling characteristics of ΔMH/MH show the potential to develop a vibrating sample magnetometer system with low field strength for clinic applications.

    Original languageEnglish
    Article number2018
    JournalSensors (Switzerland)
    Volume17
    Issue number9
    DOIs
    Publication statusPublished - 2017 Sep 3

    Keywords

    • Alpha-fetoprotein
    • Biofunctionalized magnetic nanoparticles
    • Biomarker
    • Hepatocellular carcinoma
    • Magnetic immunoassay
    • Magnetization enhancement

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry
    • Atomic and Molecular Physics, and Optics
    • Instrumentation
    • Electrical and Electronic Engineering

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