Characterization of magnetic relaxation when biofunctionalized magnetic nano-particles are associated with biomarkers in the liquid state in biomedical applications

Shu Hsien Liao, Han Sheng Huang, Jean Hong Chen, Yu Kai Su, Yuan Fu Tong

    Research output: Contribution to journalArticle

    Abstract

    This study determined the characteristics of the time-dependent effective relaxation time τeff and magnetization M when biofunctionalized magnetic nanoparticles (BMNPs) associated with biomarkers in a liquid immunoassay. Carcinoembryonic antigen (CEA) was used as the biomarker. BMNP is an anti-CEA that was coated onto dextran-coated Fe3O4 and labeled as Fe3O4-anti-CEA. The phase lag θ of M with respect to the applied field H was measured using a sensitive homemade alternative-current susceptometer, and θ was measured using lock-in detection. The results were used to estimate τeff using the relationship tanθ(t) = ωτeff(t), where 2πf = ω and f is the excitation frequency. Δτeff increased with ΦCEA, where Δτeff was the increment of τeff after Fe3O4-anti-CEA associated with CEA, and ΦCEA was the concentration of CEA. Additionally, M enhanced when ΦCEA increased. We attributed these enhancements to magnetic dipole-dipole interactions among MNPs that contributed extra M and in turn enhanced τeff. Magnetic clusters after the association were verified using a transmission electron microscope. This study established the relationship between Δτeffeff,0 and ΦCEA, where τeff,0 = τeffCEA = 0), allowing the assay of an unknown amount of CEA molecules.

    LanguageEnglish
    Pages4057-4062
    Number of pages6
    JournalRSC Advances
    Volume8
    Issue number8
    DOIs
    Publication statusPublished - 2018 Jan 1

    Fingerprint

    Magnetic relaxation
    Carcinoembryonic Antigen
    Biomarkers
    Antigens
    Liquids
    Nanoparticles
    Dextran
    Dextrans
    Relaxation time
    Assays
    Magnetization
    Electron microscopes
    Association reactions
    Molecules

    ASJC Scopus subject areas

    • Chemistry(all)
    • Chemical Engineering(all)

    Cite this

    Characterization of magnetic relaxation when biofunctionalized magnetic nano-particles are associated with biomarkers in the liquid state in biomedical applications. / Liao, Shu Hsien; Huang, Han Sheng; Chen, Jean Hong; Su, Yu Kai; Tong, Yuan Fu.

    In: RSC Advances, Vol. 8, No. 8, 01.01.2018, p. 4057-4062.

    Research output: Contribution to journalArticle

    @article{ba26f35cbe814b61a62849a8a368b777,
    title = "Characterization of magnetic relaxation when biofunctionalized magnetic nano-particles are associated with biomarkers in the liquid state in biomedical applications",
    abstract = "This study determined the characteristics of the time-dependent effective relaxation time τeff and magnetization M when biofunctionalized magnetic nanoparticles (BMNPs) associated with biomarkers in a liquid immunoassay. Carcinoembryonic antigen (CEA) was used as the biomarker. BMNP is an anti-CEA that was coated onto dextran-coated Fe3O4 and labeled as Fe3O4-anti-CEA. The phase lag θ of M with respect to the applied field H was measured using a sensitive homemade alternative-current susceptometer, and θ was measured using lock-in detection. The results were used to estimate τeff using the relationship tanθ(t) = ωτeff(t), where 2πf = ω and f is the excitation frequency. Δτeff increased with ΦCEA, where Δτeff was the increment of τeff after Fe3O4-anti-CEA associated with CEA, and ΦCEA was the concentration of CEA. Additionally, M enhanced when ΦCEA increased. We attributed these enhancements to magnetic dipole-dipole interactions among MNPs that contributed extra M and in turn enhanced τeff. Magnetic clusters after the association were verified using a transmission electron microscope. This study established the relationship between Δτeff/τeff,0 and ΦCEA, where τeff,0 = τeff (ΦCEA = 0), allowing the assay of an unknown amount of CEA molecules.",
    author = "Liao, {Shu Hsien} and Huang, {Han Sheng} and Chen, {Jean Hong} and Su, {Yu Kai} and Tong, {Yuan Fu}",
    year = "2018",
    month = "1",
    day = "1",
    doi = "10.1039/c7ra12634d",
    language = "English",
    volume = "8",
    pages = "4057--4062",
    journal = "RSC Advances",
    issn = "2046-2069",
    publisher = "Royal Society of Chemistry",
    number = "8",

    }

    TY - JOUR

    T1 - Characterization of magnetic relaxation when biofunctionalized magnetic nano-particles are associated with biomarkers in the liquid state in biomedical applications

    AU - Liao, Shu Hsien

    AU - Huang, Han Sheng

    AU - Chen, Jean Hong

    AU - Su, Yu Kai

    AU - Tong, Yuan Fu

    PY - 2018/1/1

    Y1 - 2018/1/1

    N2 - This study determined the characteristics of the time-dependent effective relaxation time τeff and magnetization M when biofunctionalized magnetic nanoparticles (BMNPs) associated with biomarkers in a liquid immunoassay. Carcinoembryonic antigen (CEA) was used as the biomarker. BMNP is an anti-CEA that was coated onto dextran-coated Fe3O4 and labeled as Fe3O4-anti-CEA. The phase lag θ of M with respect to the applied field H was measured using a sensitive homemade alternative-current susceptometer, and θ was measured using lock-in detection. The results were used to estimate τeff using the relationship tanθ(t) = ωτeff(t), where 2πf = ω and f is the excitation frequency. Δτeff increased with ΦCEA, where Δτeff was the increment of τeff after Fe3O4-anti-CEA associated with CEA, and ΦCEA was the concentration of CEA. Additionally, M enhanced when ΦCEA increased. We attributed these enhancements to magnetic dipole-dipole interactions among MNPs that contributed extra M and in turn enhanced τeff. Magnetic clusters after the association were verified using a transmission electron microscope. This study established the relationship between Δτeff/τeff,0 and ΦCEA, where τeff,0 = τeff (ΦCEA = 0), allowing the assay of an unknown amount of CEA molecules.

    AB - This study determined the characteristics of the time-dependent effective relaxation time τeff and magnetization M when biofunctionalized magnetic nanoparticles (BMNPs) associated with biomarkers in a liquid immunoassay. Carcinoembryonic antigen (CEA) was used as the biomarker. BMNP is an anti-CEA that was coated onto dextran-coated Fe3O4 and labeled as Fe3O4-anti-CEA. The phase lag θ of M with respect to the applied field H was measured using a sensitive homemade alternative-current susceptometer, and θ was measured using lock-in detection. The results were used to estimate τeff using the relationship tanθ(t) = ωτeff(t), where 2πf = ω and f is the excitation frequency. Δτeff increased with ΦCEA, where Δτeff was the increment of τeff after Fe3O4-anti-CEA associated with CEA, and ΦCEA was the concentration of CEA. Additionally, M enhanced when ΦCEA increased. We attributed these enhancements to magnetic dipole-dipole interactions among MNPs that contributed extra M and in turn enhanced τeff. Magnetic clusters after the association were verified using a transmission electron microscope. This study established the relationship between Δτeff/τeff,0 and ΦCEA, where τeff,0 = τeff (ΦCEA = 0), allowing the assay of an unknown amount of CEA molecules.

    UR - http://www.scopus.com/inward/record.url?scp=85041206610&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=85041206610&partnerID=8YFLogxK

    U2 - 10.1039/c7ra12634d

    DO - 10.1039/c7ra12634d

    M3 - Article

    VL - 8

    SP - 4057

    EP - 4062

    JO - RSC Advances

    T2 - RSC Advances

    JF - RSC Advances

    SN - 2046-2069

    IS - 8

    ER -