Characterization of genomic structures and expression profiles of three tandem repeats of a mouse double homeobox gene: Duxbl

Shey Lin Wu, Ming Shiun Tsai, Swee Hee Wong, Hsiu Mei Hsieh, Tz Shiu Tsai, Wei Tang Chang, Shin Ling Huang, Chun Ching Chiu, Sue Hong Wang

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

We identified and cloned a mouse double homeobox gene (Duxbl), which encodes two homeodomains. Duxbl gene, a tandem triplicate produces two major transcripts, Duxbl and Duxbl-s. The amino acid sequences of Duxbl homeodomains are most similar to those of human DUX4 protein, associated with facioscapulohumeral muscular dystrophy. In adult tissues, Duxbl is predominantly expressed in female reproductive organs and eyes, and slightly expressed in brain and testes. During gonad development, Duxbl is expressed from embryonic to adult stages and specifically expressed in oocytes and spermatogonia. During embryonic development, Duxbl is transcribed in limbs and tail. However, Duxbl proteins were only detected in trunk and limb muscles and in elongated myocytes and myotubes. In C2C12 muscle cell line, Duxbl expression pattern is similar to differentiated marker gene, Myogenin, increased in expression from 2 days onward in differentiating medium. We suggest that Duxbl proteins play regulatory roles during myogenesis and reproductive developments.

Original languageEnglish
Pages (from-to)927-940
Number of pages14
JournalDevelopmental Dynamics
Volume239
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1

Keywords

  • Double homeobox gene
  • Duxbl
  • Mouse embryo
  • Myogenesis
  • Reproductive development

ASJC Scopus subject areas

  • Developmental Biology

Fingerprint Dive into the research topics of 'Characterization of genomic structures and expression profiles of three tandem repeats of a mouse double homeobox gene: Duxbl'. Together they form a unique fingerprint.

Cite this