Characterization of genomic structures and expression profiles of three tandem repeats of a mouse double homeobox gene: Duxbl

Shey Lin Wu, Ming Shiun Tsai, Swee Hee Wong, Hsiu Mei Hsieh, Tz Shiu Tsai, Wei Tang Chang, Shin Ling Huang, Chun Ching Chiu, Sue Hong Wang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We identified and cloned a mouse double homeobox gene (Duxbl), which encodes two homeodomains. Duxbl gene, a tandem triplicate produces two major transcripts, Duxbl and Duxbl-s. The amino acid sequences of Duxbl homeodomains are most similar to those of human DUX4 protein, associated with facioscapulohumeral muscular dystrophy. In adult tissues, Duxbl is predominantly expressed in female reproductive organs and eyes, and slightly expressed in brain and testes. During gonad development, Duxbl is expressed from embryonic to adult stages and specifically expressed in oocytes and spermatogonia. During embryonic development, Duxbl is transcribed in limbs and tail. However, Duxbl proteins were only detected in trunk and limb muscles and in elongated myocytes and myotubes. In C2C12 muscle cell line, Duxbl expression pattern is similar to differentiated marker gene, Myogenin, increased in expression from 2 days onward in differentiating medium. We suggest that Duxbl proteins play regulatory roles during myogenesis and reproductive developments.

Original languageEnglish
Pages (from-to)927-940
Number of pages14
JournalDevelopmental Dynamics
Volume239
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1

Fingerprint

Tandem Repeat Sequences
Homeobox Genes
Muscle Cells
Extremities
Facioscapulohumeral Muscular Dystrophy
Myogenin
Spermatogonia
Muscle Development
Skeletal Muscle Fibers
Gonads
Genes
Oocytes
Embryonic Development
Tail
Testis
Amino Acid Sequence
Proteins
Cell Line
Muscles
Brain

Keywords

  • Double homeobox gene
  • Duxbl
  • Mouse embryo
  • Myogenesis
  • Reproductive development

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Characterization of genomic structures and expression profiles of three tandem repeats of a mouse double homeobox gene : Duxbl. / Wu, Shey Lin; Tsai, Ming Shiun; Wong, Swee Hee; Hsieh, Hsiu Mei; Tsai, Tz Shiu; Chang, Wei Tang; Huang, Shin Ling; Chiu, Chun Ching; Wang, Sue Hong.

In: Developmental Dynamics, Vol. 239, No. 3, 01.03.2010, p. 927-940.

Research output: Contribution to journalArticle

Wu, Shey Lin ; Tsai, Ming Shiun ; Wong, Swee Hee ; Hsieh, Hsiu Mei ; Tsai, Tz Shiu ; Chang, Wei Tang ; Huang, Shin Ling ; Chiu, Chun Ching ; Wang, Sue Hong. / Characterization of genomic structures and expression profiles of three tandem repeats of a mouse double homeobox gene : Duxbl. In: Developmental Dynamics. 2010 ; Vol. 239, No. 3. pp. 927-940.
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