Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry

Po Jui Chen; Sung Fang Chen; Yu Ju Chen

Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry

Po Jui Chen^*, Sung Fang Chen, Yu Ju Chen

^*Corresponding author for this work

Research output: Contribution to conference › Paper › peer-review

Abstract

The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and Co^II complex in 20 μL H₂O with 0.1% H₂O₂. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the Co^II complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.

Original language	English
Pages	435-436
Number of pages	2
Publication status	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2002 Jun 2 → 2002 Jun 6

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	2002/06/02 → 2002/06/06

ASJC Scopus subject areas

Spectroscopy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry. / Chen, Po Jui; Chen, Sung Fang; Chen, Yu Ju.
2002. 435-436 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

@conference{0d06da32a5ca4e8ca9bbc965bfd2a205,

title = "Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry",

abstract = "The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and CoII complex in 20 μL H2O with 0.1% H2O2. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the CoII complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.",

author = "Chen, {Po Jui} and Chen, {Sung Fang} and Chen, {Yu Ju}",

year = "2002",

language = "English",

pages = "435--436",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry

AU - Chen, Po Jui

AU - Chen, Sung Fang

AU - Chen, Yu Ju

PY - 2002

Y1 - 2002

N2 - The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and CoII complex in 20 μL H2O with 0.1% H2O2. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the CoII complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.

AB - The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and CoII complex in 20 μL H2O with 0.1% H2O2. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the CoII complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.

UR - http://www.scopus.com/inward/record.url?scp=2442640602&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442640602&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442640602

SP - 435

EP - 436

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry

Abstract

Other

ASJC Scopus subject areas

UN SDGs

Other files and links

Fingerprint

Cite this