Abstract
The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and CoII complex in 20 μL H2O with 0.1% H2O2. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the CoII complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.
| Original language | English |
|---|---|
| Pages | 435-436 |
| Number of pages | 2 |
| Publication status | Published - 2002 Dec 1 |
| Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2002 Jun 2 → 2002 Jun 6 |
Other
| Other | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
|---|---|
| Country | United States |
| City | Orlando, FL |
| Period | 02/6/2 → 02/6/6 |
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ASJC Scopus subject areas
- Spectroscopy
Cite this
Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry. / Chen, Po Jui; Chen, Sung Fang; Chen, Yu Ju.
2002. 435-436 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.Research output: Contribution to conference › Paper
}
TY - CONF
T1 - Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry
AU - Chen, Po Jui
AU - Chen, Sung Fang
AU - Chen, Yu Ju
PY - 2002/12/1
Y1 - 2002/12/1
N2 - The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and CoII complex in 20 μL H2O with 0.1% H2O2. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the CoII complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.
AB - The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and CoII complex in 20 μL H2O with 0.1% H2O2. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the CoII complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.
UR - http://www.scopus.com/inward/record.url?scp=2442640602&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442640602&partnerID=8YFLogxK
M3 - Paper
AN - SCOPUS:2442640602
SP - 435
EP - 436
ER -