Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry

Po Jui Chen, Sung Fang Chen, Yu Ju Chen

Research output: Contribution to conference › Paper

Abstract

The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and CoII complex in 20 μL H2O with 0.1% H2O2. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the CoII complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.

Original languageEnglish
Pages435-436
Number of pages2
Publication statusPublished - 2002 Dec 1
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2002 Jun 2 → 2002 Jun 6

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period02/6/2 → 02/6/6

ASJC Scopus subject areas

  • Spectroscopy

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    Chen, P. J., Chen, S. F., & Chen, Y. J. (2002). Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry. 435-436. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.