Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry

Po Jui Chen; Sung Fang Chen; Yu Ju Chen

Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry

Po Jui Chen, Sung Fang Chen, Yu Ju Chen

Department of Chemistry

Research output: Contribution to conference › Paper › peer-review

Abstract

The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and Co^II complex in 20 μL H₂O with 0.1% H₂O₂. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the Co^II complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.

Original language	English
Pages	435-436
Number of pages	2
Publication status	Published - 2002 Dec 1
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2002 Jun 2 → 2002 Jun 6

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country	United States
City	Orlando, FL
Period	2002/06/02 → 2002/06/06

ASJC Scopus subject areas

Spectroscopy

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Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry. / Chen, Po Jui; Chen, Sung Fang; Chen, Yu Ju.

2002. 435-436 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

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abstract = "The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and CoII complex in 20 μL H2O with 0.1% H2O2. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the CoII complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.",

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N2 - The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and CoII complex in 20 μL H2O with 0.1% H2O2. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the CoII complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.

AB - The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and CoII complex in 20 μL H2O with 0.1% H2O2. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the CoII complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.

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