Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry

Po Jui Chen, Sung Fang Chen, Yu Ju Chen

Research output: Contribution to conferencePaperpeer-review

Abstract

The determination of reaction mechanism of DNA cleavage using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was discussed. The cleavage of HIV-27 was done by mixing 20 μM of HIV-27 and CoII complex in 20 μL H2O with 0.1% H2O2. The purification of HIV-27 and its cleavage products was done by ethanol precipitation, followed by denaturation. It was observed that the CoII complex was a bulge-specific probe to DNA structure, cleavaging at bulge site.

Original languageEnglish
Pages435-436
Number of pages2
Publication statusPublished - 2002 Dec 1
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2002 Jun 22002 Jun 6

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period2002/06/022002/06/06

ASJC Scopus subject areas

  • Spectroscopy

Fingerprint Dive into the research topics of 'Characterization of bulge-specific DNA cleaver by matrix-assisted laser desorption inization time-of-flight mass spectrometry'. Together they form a unique fingerprint.

Cite this