Characterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cells

Chen Wen Lu, Jong Kai Hsiao, Hon Man Liu, Chung Hsin Wu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The aim of the current study was to develop an iron oxide nanoparticle (ION) labelling and magnetic resonance imaging (MRI)-based protocol to allow visualization of the differentiation process of mesenchymal stem cells (MSCs) into neural-like cells (NCs) in vitro. Ferucarbotran, a clinically available ION, which can be visualized under MRI, is used for tracking cells implanted in vivo. The NCs were verified morphologically and histologically by light microscopy, and their functions were verified by measuring their action potentials. Conformational conversion of axon-like structures was observed under light microscopy. These NCs exhibited frequent, active action potentials compared with cells that did not undergo neural differentiation. The labelling of ION had no influence on the morphological and functional differentiation capacity of the MSCs. We conclude that the MSCs that were differentiated into NCs exhibited in vitro activity potential firing and may be used to replace damaged neurons.

Original languageEnglish
Article number3587
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Mesenchymal Stromal Cells
Nanoparticles
Magnetic Resonance Imaging
Action Potentials
Microscopy
Cell Tracking
Light
Axons
ferric oxide
Neurons
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Characterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cells. / Lu, Chen Wen; Hsiao, Jong Kai; Liu, Hon Man; Wu, Chung Hsin.

In: Scientific Reports, Vol. 7, No. 1, 3587, 01.12.2017.

Research output: Contribution to journalArticle

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