Abstract
Premise of the study: Transferable polymorphic microsatellite loci for four skullcaps, Scutellaria indica, S. taiwanensis, S. austrotaiwanensis, and S. playfairii, were developed for future studies of the mating system and population structure of these species. Interspecific amplification was also tested in various Scutellaria species. Methods and Results: Twelve novel polymorphic microsatellite loci were isolated from four S. taiwanensis specimens, and seven are interspecifically transferable. Microsatellite loci developed from S. austrotaiwanensis in a previous study were also analyzed in the other three species, and 12 loci were found to be transferable. Allele numbers of the total 24 loci for S. indica, S. taiwanensis, S. playfairii, and S. austrotaiwanensis are two to four, two, two to five, and two to three, respectively, with an expected heterozygosity ranging from 0.114-0.661, 0.062-0.499, 0.280-0.730, and 0.268-0.662, respectively. The interspecies transferability of these 24 loci was further tested in another 10 Scutellaria species, including three species native to Taiwan. Seventeen loci were found to be interspecifically amplifiable, especially among the Taiwan native species. Conclusions: These highly polymorphic and transferable loci will be useful for future studies of the mating system of closely related Scutellaria species.
| Original language | English |
|---|---|
| Pages (from-to) | e24-e27 |
| Journal | American Journal of Botany |
| Volume | 99 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2012 Jan 1 |
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Keywords
- Dimorphic cleistogamy
- Interspecific transferability
- Labiatae
- Mating system
- Scutellaria
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Genetics
- Plant Science
Cite this
Characterization of 24 transferable microsatellite loci in four skullcaps (scutellaria, labiatae). / Chiang, Yu Chung; Huang, Bing Hong; Shih, Huei Chuan; Hsu, Tsai Wen; Chang, Chun Wen; Liao, Pei Chun.
In: American Journal of Botany, Vol. 99, No. 1, 01.01.2012, p. e24-e27.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of 24 transferable microsatellite loci in four skullcaps (scutellaria, labiatae)
AU - Chiang, Yu Chung
AU - Huang, Bing Hong
AU - Shih, Huei Chuan
AU - Hsu, Tsai Wen
AU - Chang, Chun Wen
AU - Liao, Pei Chun
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Premise of the study: Transferable polymorphic microsatellite loci for four skullcaps, Scutellaria indica, S. taiwanensis, S. austrotaiwanensis, and S. playfairii, were developed for future studies of the mating system and population structure of these species. Interspecific amplification was also tested in various Scutellaria species. Methods and Results: Twelve novel polymorphic microsatellite loci were isolated from four S. taiwanensis specimens, and seven are interspecifically transferable. Microsatellite loci developed from S. austrotaiwanensis in a previous study were also analyzed in the other three species, and 12 loci were found to be transferable. Allele numbers of the total 24 loci for S. indica, S. taiwanensis, S. playfairii, and S. austrotaiwanensis are two to four, two, two to five, and two to three, respectively, with an expected heterozygosity ranging from 0.114-0.661, 0.062-0.499, 0.280-0.730, and 0.268-0.662, respectively. The interspecies transferability of these 24 loci was further tested in another 10 Scutellaria species, including three species native to Taiwan. Seventeen loci were found to be interspecifically amplifiable, especially among the Taiwan native species. Conclusions: These highly polymorphic and transferable loci will be useful for future studies of the mating system of closely related Scutellaria species.
AB - Premise of the study: Transferable polymorphic microsatellite loci for four skullcaps, Scutellaria indica, S. taiwanensis, S. austrotaiwanensis, and S. playfairii, were developed for future studies of the mating system and population structure of these species. Interspecific amplification was also tested in various Scutellaria species. Methods and Results: Twelve novel polymorphic microsatellite loci were isolated from four S. taiwanensis specimens, and seven are interspecifically transferable. Microsatellite loci developed from S. austrotaiwanensis in a previous study were also analyzed in the other three species, and 12 loci were found to be transferable. Allele numbers of the total 24 loci for S. indica, S. taiwanensis, S. playfairii, and S. austrotaiwanensis are two to four, two, two to five, and two to three, respectively, with an expected heterozygosity ranging from 0.114-0.661, 0.062-0.499, 0.280-0.730, and 0.268-0.662, respectively. The interspecies transferability of these 24 loci was further tested in another 10 Scutellaria species, including three species native to Taiwan. Seventeen loci were found to be interspecifically amplifiable, especially among the Taiwan native species. Conclusions: These highly polymorphic and transferable loci will be useful for future studies of the mating system of closely related Scutellaria species.
KW - Dimorphic cleistogamy
KW - Interspecific transferability
KW - Labiatae
KW - Mating system
KW - Scutellaria
UR - http://www.scopus.com/inward/record.url?scp=84863387346&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863387346&partnerID=8YFLogxK
U2 - 10.3732/ajb.1100279
DO - 10.3732/ajb.1100279
M3 - Article
C2 - 22203648
AN - SCOPUS:84863387346
VL - 99
SP - e24-e27
JO - American Journal of Botany
JF - American Journal of Botany
SN - 0002-9122
IS - 1
ER -