Characterization and toxicology evaluation of low molecular weight chitosan on zebrafish

Chih Ming Chou, Fwu Long Mi, Jiun Lin Horng, Li Yih Lin, Min Lang Tsai, Chao Lin Liu, Kun Ying Lu, Cheng Ying Chu, Yu Tzu Chen, Yu Lin A. Lee, Chia Hsiung Cheng*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Chitosan is suggested as no or low toxicity and biocompatible biomaterial. Digestion of chitosan to reduce molecular weight and formulate nanoparticle was generally used to improve efficiency for DNA or protein delivery. However, the toxicity of low-molecular-weight chitosan (LMWCS) towards freshwater fishes has not been well evaluated. Here, we reported the toxic mechanism of LMWCS using zebrafish (Danio rerio) liver (ZFL) cell line, zebrafish larvae, and adult fish. LMWCS rapidly induced cytotoxicity of ZFL cells and death of zebrafish. Cell membrane damaged by LMWCS reduced cell viability. Damaged membrane of epithelial cell in zebrafish larvae induced breakage of the yolk. Adult fish exhibited hypoxia before death due to multiple damages induced by LMWCS. Although the toxicity of LMWCS was revealed in zebrafish model, the toxicity was only present in pH < 7 and easy be neutralized by other negative ions. Collectively, these data improved a new understanding of LMWCS properties.

Original languageEnglish
Article number116164
JournalCarbohydrate Polymers
Publication statusPublished - 2020 Jul 15


  • Low-molecular-weight chitosan
  • Nanoparticle
  • Toxicity
  • Zebrafish

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry


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