Characterization and the broad cross-species applicability of 20 anonymous nuclear loci isolated from the Taiwan Hwamei (Garrulax taewanus)

Rong Chien Lin, Cheng Te Yao, Wen Sui Lo, Shou Hsien Li

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We isolated 20 anonymous nuclear loci (8556 bp in total) from the Taiwan Hwamei (Garrulax taewanus), an endemic songbird of Taiwan. A panel of nine to 15 individuals with unknown relationship was used to characterize polymorphism of these loci. We identified 46 single nucleotide polymorphic sites (SNPs) in 15 polymorphic loci. Frequency of SNPs was one per every 186 bp in average. Nucleotide diversity, θ, ranged from 0.00054 to 0.00371 per locus. We also tested cross-species applicability of these loci on 17 species from eight different passerine families. All 20 loci could be successfully amplified (ranged from one to 16 species, mean = 7.9 species).

Original languageEnglish
Pages (from-to)156-159
Number of pages4
JournalMolecular Ecology Notes
Volume7
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Taiwan
Single Nucleotide Polymorphism
Nucleotides
loci
Songbirds
Polymorphism
songbird
passerine
nucleotides
polymorphism
songbirds
Garrulax taewanus

Keywords

  • Anonymous nuclear loci
  • Garrulax taewanus
  • Hybridization
  • Single nucleotide polymorphism

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Characterization and the broad cross-species applicability of 20 anonymous nuclear loci isolated from the Taiwan Hwamei (Garrulax taewanus). / Lin, Rong Chien; Yao, Cheng Te; Lo, Wen Sui; Li, Shou Hsien.

In: Molecular Ecology Notes, Vol. 7, No. 1, 01.01.2007, p. 156-159.

Research output: Contribution to journalArticle

@article{c83217a5856048a190ca5b3fca85a139,
title = "Characterization and the broad cross-species applicability of 20 anonymous nuclear loci isolated from the Taiwan Hwamei (Garrulax taewanus)",
abstract = "We isolated 20 anonymous nuclear loci (8556 bp in total) from the Taiwan Hwamei (Garrulax taewanus), an endemic songbird of Taiwan. A panel of nine to 15 individuals with unknown relationship was used to characterize polymorphism of these loci. We identified 46 single nucleotide polymorphic sites (SNPs) in 15 polymorphic loci. Frequency of SNPs was one per every 186 bp in average. Nucleotide diversity, θ, ranged from 0.00054 to 0.00371 per locus. We also tested cross-species applicability of these loci on 17 species from eight different passerine families. All 20 loci could be successfully amplified (ranged from one to 16 species, mean = 7.9 species).",
keywords = "Anonymous nuclear loci, Garrulax taewanus, Hybridization, Single nucleotide polymorphism",
author = "Lin, {Rong Chien} and Yao, {Cheng Te} and Lo, {Wen Sui} and Li, {Shou Hsien}",
year = "2007",
month = "1",
day = "1",
doi = "10.1111/j.1471-8286.2006.01564.x",
language = "English",
volume = "7",
pages = "156--159",
journal = "Molecular Ecology Resources",
issn = "1755-098X",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Characterization and the broad cross-species applicability of 20 anonymous nuclear loci isolated from the Taiwan Hwamei (Garrulax taewanus)

AU - Lin, Rong Chien

AU - Yao, Cheng Te

AU - Lo, Wen Sui

AU - Li, Shou Hsien

PY - 2007/1/1

Y1 - 2007/1/1

N2 - We isolated 20 anonymous nuclear loci (8556 bp in total) from the Taiwan Hwamei (Garrulax taewanus), an endemic songbird of Taiwan. A panel of nine to 15 individuals with unknown relationship was used to characterize polymorphism of these loci. We identified 46 single nucleotide polymorphic sites (SNPs) in 15 polymorphic loci. Frequency of SNPs was one per every 186 bp in average. Nucleotide diversity, θ, ranged from 0.00054 to 0.00371 per locus. We also tested cross-species applicability of these loci on 17 species from eight different passerine families. All 20 loci could be successfully amplified (ranged from one to 16 species, mean = 7.9 species).

AB - We isolated 20 anonymous nuclear loci (8556 bp in total) from the Taiwan Hwamei (Garrulax taewanus), an endemic songbird of Taiwan. A panel of nine to 15 individuals with unknown relationship was used to characterize polymorphism of these loci. We identified 46 single nucleotide polymorphic sites (SNPs) in 15 polymorphic loci. Frequency of SNPs was one per every 186 bp in average. Nucleotide diversity, θ, ranged from 0.00054 to 0.00371 per locus. We also tested cross-species applicability of these loci on 17 species from eight different passerine families. All 20 loci could be successfully amplified (ranged from one to 16 species, mean = 7.9 species).

KW - Anonymous nuclear loci

KW - Garrulax taewanus

KW - Hybridization

KW - Single nucleotide polymorphism

UR - http://www.scopus.com/inward/record.url?scp=33845985944&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845985944&partnerID=8YFLogxK

U2 - 10.1111/j.1471-8286.2006.01564.x

DO - 10.1111/j.1471-8286.2006.01564.x

M3 - Article

AN - SCOPUS:33845985944

VL - 7

SP - 156

EP - 159

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

SN - 1755-098X

IS - 1

ER -