Characterization and the broad cross-species applicability of 20 anonymous nuclear loci isolated from the Taiwan Hwamei (Garrulax taewanus)

Rong Chien Lin, Cheng Te Yao, Wen Sui Lo, Shou Hsien Li

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We isolated 20 anonymous nuclear loci (8556 bp in total) from the Taiwan Hwamei (Garrulax taewanus), an endemic songbird of Taiwan. A panel of nine to 15 individuals with unknown relationship was used to characterize polymorphism of these loci. We identified 46 single nucleotide polymorphic sites (SNPs) in 15 polymorphic loci. Frequency of SNPs was one per every 186 bp in average. Nucleotide diversity, θ, ranged from 0.00054 to 0.00371 per locus. We also tested cross-species applicability of these loci on 17 species from eight different passerine families. All 20 loci could be successfully amplified (ranged from one to 16 species, mean = 7.9 species).

Original languageEnglish
Pages (from-to)156-159
Number of pages4
JournalMolecular Ecology Notes
Volume7
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

Keywords

  • Anonymous nuclear loci
  • Garrulax taewanus
  • Hybridization
  • Single nucleotide polymorphism

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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