Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles

Kai Wen Huang, Jen Jie Chieh, Herng Er Horng, Chin Yih Hong, Hong Chang Yang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

For preoperative and intraoperative detection of tumor distribution, numerous multimodal contrast agents, such as magnetic nanoparticles (MNPs) with several examination indicators, are currently in development. However, complex materials, configuration, and cost are required for multimodal contrast agents, accompanied by a high possibility of toxicity and low popularity in clinics. Nevertheless, the magnetic labeling of MNPs using bioprobes should be feasible not only in preoperative magnetic resonance imaging (MRI), but also in intraoperative examination based on other magnetic properties. In this study, anti-alpha-fetoprotein (AFP)-mediated Fe3O4 MNPs, injected into mice with liver tumors, were used to examine the characteristics of magnetic labeling. Using MRI and scanning superconducting-quantum-interference-device biosusceptometry (SSB), based on alternating current (AC) susceptibility, the magnetic labeling occurred significantly on the first day post-injection of anti-AFP magnetic fluid (MF), and then decreased over time. However, for both MF without antibodies and an anti-carcinoembryonic antigen MF, no magnetic labeling occured on the first day of their respective post-injection. The favorable agreement indicates that magnetic labeling possesses two magnetic characteristics: distortion of the imaging field and AC susceptibility. In addition, the results of the biopsy tests, anti-AFP staining, and Prussian blue staining show the same dynamics as those of magnetic methodologies and prove that bound MNPs on tumor tissue are rotatable by an AC magnetic field to express AC susceptibility. Therefore, with the simple configuration of antibody-mediated MNPs, magnetic labeling is also feasible for intraoperative examinations using SSB with high mobility and sensitivity.

Original languageEnglish
Pages (from-to)2987-2996
Number of pages10
JournalInternational Journal of Nanomedicine
Volume7
DOIs
Publication statusPublished - 2012 Dec 5

Fingerprint

alpha-Fetoproteins
Liver
Nanoparticles
Labeling
Tumors
Magnetic fluids
Neoplasms
Contrast Media
Magnetic resonance
Imaging techniques
Antibodies
Magnetic Resonance Imaging
Staining and Labeling
Injections
Carcinoembryonic Antigen
Biosensing Techniques
Magnetic Fields
Biopsy
SQUIDs
Antigens

Keywords

  • Alpha-fetoprotein
  • Magnetic resonance imaging
  • Scanning SQUID biosusceptometry

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

Cite this

Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles. / Huang, Kai Wen; Chieh, Jen Jie; Horng, Herng Er; Hong, Chin Yih; Yang, Hong Chang.

In: International Journal of Nanomedicine, Vol. 7, 05.12.2012, p. 2987-2996.

Research output: Contribution to journalArticle

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