Cellular distributions of creatine kinase in branchia of euryhaline tilapia (Oreochromis mossambicus)

Li-Yih Lin, Chia Chang Chiang, Hong Yi Gong, Ching Yi Cheng, Pung Pung Hwang, Ching Feng Weng

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Although euryhaline teleosts can adapt to environmental fluctuation of salinity, their energy source for responding to changes in salinity and osmolarity remains unclear. This study examines the cellular localization of creatine kinase (CK) expression in branchia of tilapia (Oreochromis mossambicus). Western blot analysis of muscle-type CK (MM form) revealed a high association with salinity changes, but BB and MB forms of CK in the gills of fish adapted to seawater did not change. With the use of immunocytochemistry, three CK isoforms (MM, MB, and BB) were localized in mitochondria-rich (MR) cells and other epithelial cells of tilapia gills. In addition, staining intensity of MM-form CK in MR cells increased after seawater transfer, whereas BB and MB forms did not significantly change. To our knowledge, this work presents the first evidence of CK expression in MR cells of tilapia gills, highlighting the potential role of CK in providing energy for ion transport.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume284
Issue number1 53-1
Publication statusPublished - 2003 Jan 1

Fingerprint

Tilapia
MM Form Creatine Kinase
Creatine Kinase
Mitochondria
Salinity
Seawater
BB Form Creatine Kinase
MB Form Creatine Kinase
Ion Transport
Osmolar Concentration
Fish
Protein Isoforms
Fishes
Western Blotting
Epithelial Cells
Immunohistochemistry
Ions
Staining and Labeling

Keywords

  • Creatine kinase isoform
  • Gill
  • Mitochondria-rich cells

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Cellular distributions of creatine kinase in branchia of euryhaline tilapia (Oreochromis mossambicus). / Lin, Li-Yih; Chiang, Chia Chang; Gong, Hong Yi; Cheng, Ching Yi; Hwang, Pung Pung; Weng, Ching Feng.

In: American Journal of Physiology - Cell Physiology, Vol. 284, No. 1 53-1, 01.01.2003.

Research output: Contribution to journalArticle

Lin, Li-Yih ; Chiang, Chia Chang ; Gong, Hong Yi ; Cheng, Ching Yi ; Hwang, Pung Pung ; Weng, Ching Feng. / Cellular distributions of creatine kinase in branchia of euryhaline tilapia (Oreochromis mossambicus). In: American Journal of Physiology - Cell Physiology. 2003 ; Vol. 284, No. 1 53-1.
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