Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine

Kai Hong Guo; Kun Hua Lu; Yi Chun Yeh

doi:10.1021/acssynbio.8b00416

Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine

Kai Hong Guo, Kun Hua Lu, Yi Chun Yeh^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.

Original language	English
Pages (from-to)	2790-2795
Number of pages	6
Journal	ACS Synthetic Biology
Volume	7
Issue number	12
DOIs	https://doi.org/10.1021/acssynbio.8b00416
Publication status	Published - 2018 Nov 12

Keywords

aromatic acids
auxin
E. coli
FeaR regulon
phenylacetic acid
phenylethylamine

ASJC Scopus subject areas

Biomedical Engineering
Biochemistry, Genetics and Molecular Biology (miscellaneous)

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10.1021/acssynbio.8b00416

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title = "Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine",

abstract = "Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.",

keywords = "aromatic acids, auxin, E. coli, FeaR regulon, phenylacetic acid, phenylethylamine",

author = "Guo, {Kai Hong} and Lu, {Kun Hua} and Yeh, {Yi Chun}",

note = "Funding Information: This work was funded by the Ministry of Science and Technology of Taiwan under the Project No. 105-2113-M-003-013-MY2 and 107-2113-M-003-013-MY3. We thank Molecular Imaging Core Facility of National Taiwan Normal University for the assistance in high-content fluorescence microscopes. Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

year = "2018",

month = nov,

day = "12",

doi = "10.1021/acssynbio.8b00416",

language = "English",

volume = "7",

pages = "2790--2795",

journal = "ACS Synthetic Biology",

issn = "2161-5063",

publisher = "American Chemical Society",

number = "12",

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T1 - Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine

AU - Guo, Kai Hong

AU - Lu, Kun Hua

AU - Yeh, Yi Chun

N1 - Funding Information: This work was funded by the Ministry of Science and Technology of Taiwan under the Project No. 105-2113-M-003-013-MY2 and 107-2113-M-003-013-MY3. We thank Molecular Imaging Core Facility of National Taiwan Normal University for the assistance in high-content fluorescence microscopes. Publisher Copyright: © 2018 American Chemical Society.

PY - 2018/11/12

Y1 - 2018/11/12

N2 - Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.

AB - Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.

KW - aromatic acids

KW - auxin

KW - E. coli

KW - FeaR regulon

KW - phenylacetic acid

KW - phenylethylamine

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U2 - 10.1021/acssynbio.8b00416

DO - 10.1021/acssynbio.8b00416

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SN - 2161-5063

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JO - ACS Synthetic Biology

JF - ACS Synthetic Biology

IS - 12

ER -

Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine

Abstract

Keywords

ASJC Scopus subject areas

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