Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine

Kai Hong Guo, Kun Hua Lu, Yi Chun Yeh

Research output: Contribution to journalArticle

Abstract

Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.

Original languageEnglish
Pages (from-to)2790-2795
Number of pages6
JournalACS Synthetic Biology
Volume7
Issue number12
DOIs
Publication statusPublished - 2018 Nov 12

Fingerprint

Phenethylamines
Biosensing Techniques
Biosensors
Acids
Regulon
Indoleacetic Acids
Plant Growth Regulators
Aromatic compounds
Biosynthesis
Hormones
Fear
Amines
phenylacetic acid

Keywords

  • aromatic acids
  • auxin
  • E. coli
  • FeaR regulon
  • phenylacetic acid
  • phenylethylamine

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine. / Guo, Kai Hong; Lu, Kun Hua; Yeh, Yi Chun.

In: ACS Synthetic Biology, Vol. 7, No. 12, 12.11.2018, p. 2790-2795.

Research output: Contribution to journalArticle

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