TY - JOUR
T1 - Capillary electrophoretic determination of the constituents of Paeoniae radix
AU - Wu, Hsin Kai
AU - Sheu, Shuenn Jyi
N1 - Funding Information:
Financial support from the National Science Council, Republic of China, is gratefully acknowledged (NSC 85-2113-M-003-008).
PY - 1996/11/8
Y1 - 1996/11/8
N2 - A method combining the techniques of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) has been developed to separate a total of eight peony constituents. The CZE method was used to determine the content of paeonol, oxypaeoniflorin, benzoic acid, pentagalloylglucose and gallic acid, and MEKC technique based on sodium cholate was applied to analyze albiflorin, paeoniflorin, benzoylalbiflorin, paeonol and oxypaeoniflorin. Linearity around two orders of magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 2.6-23.7 μg/ml. The relative standard deviations of migration times were less than 1.43% (n = 6). Contents of peony constituents in an ethanol-water extract of Paeonia lactiflora Pall. sample could easily be determined by this method.
AB - A method combining the techniques of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) has been developed to separate a total of eight peony constituents. The CZE method was used to determine the content of paeonol, oxypaeoniflorin, benzoic acid, pentagalloylglucose and gallic acid, and MEKC technique based on sodium cholate was applied to analyze albiflorin, paeoniflorin, benzoylalbiflorin, paeonol and oxypaeoniflorin. Linearity around two orders of magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 2.6-23.7 μg/ml. The relative standard deviations of migration times were less than 1.43% (n = 6). Contents of peony constituents in an ethanol-water extract of Paeonia lactiflora Pall. sample could easily be determined by this method.
KW - Paeniae Radix
KW - Peony constituents
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U2 - 10.1016/S0021-9673(96)00525-0
DO - 10.1016/S0021-9673(96)00525-0
M3 - Article
C2 - 8962508
AN - SCOPUS:0030575858
SN - 0021-9673
VL - 753
SP - 139
EP - 146
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1
ER -