Capillary electrophoretic determination of the constituents of Paeoniae radix

Hsin Kai Wu; Shuenn Jyi Sheu

doi:10.1016/S0021-9673(96)00525-0

Capillary electrophoretic determination of the constituents of Paeoniae radix

Hsin Kai Wu, Shuenn Jyi Sheu

Graduate Institute of Science Education

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

A method combining the techniques of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) has been developed to separate a total of eight peony constituents. The CZE method was used to determine the content of paeonol, oxypaeoniflorin, benzoic acid, pentagalloylglucose and gallic acid, and MEKC technique based on sodium cholate was applied to analyze albiflorin, paeoniflorin, benzoylalbiflorin, paeonol and oxypaeoniflorin. Linearity around two orders of magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 2.6-23.7 μg/ml. The relative standard deviations of migration times were less than 1.43% (n = 6). Contents of peony constituents in an ethanol-water extract of Paeonia lactiflora Pall. sample could easily be determined by this method.

Original language	English
Pages (from-to)	139-146
Number of pages	8
Journal	Journal of Chromatography A
Volume	753
Issue number	1
DOIs	https://doi.org/10.1016/S0021-9673(96)00525-0
Publication status	Published - 1996 Nov 8

Keywords

Paeniae Radix
Peony constituents

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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title = "Capillary electrophoretic determination of the constituents of Paeoniae radix",

abstract = "A method combining the techniques of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) has been developed to separate a total of eight peony constituents. The CZE method was used to determine the content of paeonol, oxypaeoniflorin, benzoic acid, pentagalloylglucose and gallic acid, and MEKC technique based on sodium cholate was applied to analyze albiflorin, paeoniflorin, benzoylalbiflorin, paeonol and oxypaeoniflorin. Linearity around two orders of magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 2.6-23.7 μg/ml. The relative standard deviations of migration times were less than 1.43% (n = 6). Contents of peony constituents in an ethanol-water extract of Paeonia lactiflora Pall. sample could easily be determined by this method.",

keywords = "Paeniae Radix, Peony constituents",

author = "Wu, {Hsin Kai} and Sheu, {Shuenn Jyi}",

note = "Funding Information: Financial support from the National Science Council, Republic of China, is gratefully acknowledged (NSC 85-2113-M-003-008).",

year = "1996",

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doi = "10.1016/S0021-9673(96)00525-0",

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T1 - Capillary electrophoretic determination of the constituents of Paeoniae radix

AU - Wu, Hsin Kai

AU - Sheu, Shuenn Jyi

N1 - Funding Information: Financial support from the National Science Council, Republic of China, is gratefully acknowledged (NSC 85-2113-M-003-008).

PY - 1996/11/8

Y1 - 1996/11/8

N2 - A method combining the techniques of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) has been developed to separate a total of eight peony constituents. The CZE method was used to determine the content of paeonol, oxypaeoniflorin, benzoic acid, pentagalloylglucose and gallic acid, and MEKC technique based on sodium cholate was applied to analyze albiflorin, paeoniflorin, benzoylalbiflorin, paeonol and oxypaeoniflorin. Linearity around two orders of magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 2.6-23.7 μg/ml. The relative standard deviations of migration times were less than 1.43% (n = 6). Contents of peony constituents in an ethanol-water extract of Paeonia lactiflora Pall. sample could easily be determined by this method.

AB - A method combining the techniques of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) has been developed to separate a total of eight peony constituents. The CZE method was used to determine the content of paeonol, oxypaeoniflorin, benzoic acid, pentagalloylglucose and gallic acid, and MEKC technique based on sodium cholate was applied to analyze albiflorin, paeoniflorin, benzoylalbiflorin, paeonol and oxypaeoniflorin. Linearity around two orders of magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 2.6-23.7 μg/ml. The relative standard deviations of migration times were less than 1.43% (n = 6). Contents of peony constituents in an ethanol-water extract of Paeonia lactiflora Pall. sample could easily be determined by this method.

KW - Paeniae Radix

KW - Peony constituents

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