Capillary electrophoretic determination of the constituents of Paeoniae radix

Hsin Kai Wu, Shuenn Jyi Sheu

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A method combining the techniques of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) has been developed to separate a total of eight peony constituents. The CZE method was used to determine the content of paeonol, oxypaeoniflorin, benzoic acid, pentagalloylglucose and gallic acid, and MEKC technique based on sodium cholate was applied to analyze albiflorin, paeoniflorin, benzoylalbiflorin, paeonol and oxypaeoniflorin. Linearity around two orders of magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 2.6-23.7 μg/ml. The relative standard deviations of migration times were less than 1.43% (n = 6). Contents of peony constituents in an ethanol-water extract of Paeonia lactiflora Pall. sample could easily be determined by this method.

Original languageEnglish
Pages (from-to)139-146
Number of pages8
JournalJournal of Chromatography A
Volume753
Issue number1
DOIs
Publication statusPublished - 1996 Nov 8

Keywords

  • Paeniae Radix
  • Peony constituents

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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