TY - JOUR
T1 - Caffeic acid improves memory impairment and brain glucose metabolism via ameliorating cerebral insulin and leptin signaling pathways in high-fat diet-induced hyperinsulinemic rats
AU - Chang, Wen Chang
AU - Kuo, Po Ling
AU - Chen, Chen Wen
AU - Wu, James Swi Bea
AU - Shen, Szu Chuan
PY - 2015/11/1
Y1 - 2015/11/1
N2 - This study investigated the effect of phenolic acid on memory decline and cerebral carbohydrate metabolism in rats fed a high-fat diet (HFD), which induced hyperinsulinemia. First, among eleven tested phenolic acids, caffeic acid (CA) was demonstrated to induce the highest glucose uptake capacity in insulin-resistant Neuro 2a mouse neuroblastoma cells. Next, male rats fed a HFD were treated with daily oral CA (30mg/kg body weight) for 30weeks. Cognitive performance trials were conducted to investigate the effect of CA on ameliorating memory impairment. Cerebral tissues were analyzed to determine whether CA affected brain carbohydrate metabolism and/or alleviated insulin and leptin resistance. Our results suggested that CA significantly reduced plasma glucose and insulin levels, and it alleviated insulin resistance (HOMA-IR) in HFD-induced hyperinsulinemic rats. Concurrently, passive avoidance tests revealed that orally administered CA significantly improved memory impairments in HFD rats. Western blot analysis demonstrated that CA significantly increased the expression of insulin signaling-related proteins, including the insulin receptor, phosphatidylinositol-3-kinase, Akt/protein kinase B, and insulin degrading enzyme. CA also increased the expression of leptin signaling-related proteins, including leptin receptor and pJAK2Tyr813/JAK2 in the cortex of HFD rats. These findings demonstrated that CA showed promise as a drug or food supplement for preventing the progression of dementia.
AB - This study investigated the effect of phenolic acid on memory decline and cerebral carbohydrate metabolism in rats fed a high-fat diet (HFD), which induced hyperinsulinemia. First, among eleven tested phenolic acids, caffeic acid (CA) was demonstrated to induce the highest glucose uptake capacity in insulin-resistant Neuro 2a mouse neuroblastoma cells. Next, male rats fed a HFD were treated with daily oral CA (30mg/kg body weight) for 30weeks. Cognitive performance trials were conducted to investigate the effect of CA on ameliorating memory impairment. Cerebral tissues were analyzed to determine whether CA affected brain carbohydrate metabolism and/or alleviated insulin and leptin resistance. Our results suggested that CA significantly reduced plasma glucose and insulin levels, and it alleviated insulin resistance (HOMA-IR) in HFD-induced hyperinsulinemic rats. Concurrently, passive avoidance tests revealed that orally administered CA significantly improved memory impairments in HFD rats. Western blot analysis demonstrated that CA significantly increased the expression of insulin signaling-related proteins, including the insulin receptor, phosphatidylinositol-3-kinase, Akt/protein kinase B, and insulin degrading enzyme. CA also increased the expression of leptin signaling-related proteins, including leptin receptor and pJAK2Tyr813/JAK2 in the cortex of HFD rats. These findings demonstrated that CA showed promise as a drug or food supplement for preventing the progression of dementia.
KW - Caffeic acid
KW - Cerebral carbohydrate metabolism
KW - Dementia
KW - Hyperinsulinemia
KW - Memory decline
UR - http://www.scopus.com/inward/record.url?scp=84943661003&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84943661003&partnerID=8YFLogxK
U2 - 10.1016/j.foodres.2015.04.010
DO - 10.1016/j.foodres.2015.04.010
M3 - Article
AN - SCOPUS:84943661003
VL - 77
SP - 24
EP - 33
JO - Food Research International
JF - Food Research International
SN - 0963-9969
ER -