Bumetanide administration attenuated traumatic brain injury through IL-1 overexpression

Kwok Tung Lu, Chang Yen Wu, Hao Han Yen, Jeng Hsiung F. Peng, Chi Ling Wang, Yi Ling Yang

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Objective: To examine the effects of administration of bumetanide, a specific NKCC1 inhibitor, on traumatic brain injury (TBI)-induced interleukin-1 (IL-1) expression. Methods: TBI model was induced by the calibrated weight drop device (450 g in weight, 2.0 m in height) in adult rats based on procedures previously reported. One hundred and sixty Wistar rats were divided into sham-control group and experimental group for time course works of TBI. The expression of IL-1β brain edema and neuronal damage were determined in these animals after TBI. Results: We found that both mRNA and protein of IL-1β were up-regulated in the hippocampus 3-24 hours after TBI. Animals displayed severe brain edema and neuron damage after TBI. Bumetanide (15 mg/kg), a specific Na+ -K+ -2Cl- cotransporter inhibitor, significantly attenuated the TBI-induced neuronal damage by IL-1β overexpression. The present stuay suggests that administration of bumetanide could significantly decreased TBI-induced inflammatory response and neuronal damage.

Original languageEnglish
Pages (from-to)404-409
Number of pages6
JournalNeurological Research
Volume29
Issue number4
DOIs
Publication statusPublished - 2007 Jun 1

Fingerprint

Bumetanide
Interleukin-1
Brain Edema
Weights and Measures
Traumatic Brain Injury
Wistar Rats
Hippocampus
Neurons
Equipment and Supplies
Control Groups
Messenger RNA

Keywords

  • IL-1
  • Na -K -2Cl -cotransporter
  • Traumatic brain injury

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Cite this

Bumetanide administration attenuated traumatic brain injury through IL-1 overexpression. / Lu, Kwok Tung; Wu, Chang Yen; Yen, Hao Han; Peng, Jeng Hsiung F.; Wang, Chi Ling; Yang, Yi Ling.

In: Neurological Research, Vol. 29, No. 4, 01.06.2007, p. 404-409.

Research output: Contribution to journalArticle

Lu, Kwok Tung ; Wu, Chang Yen ; Yen, Hao Han ; Peng, Jeng Hsiung F. ; Wang, Chi Ling ; Yang, Yi Ling. / Bumetanide administration attenuated traumatic brain injury through IL-1 overexpression. In: Neurological Research. 2007 ; Vol. 29, No. 4. pp. 404-409.
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AU - Yang, Yi Ling

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