Brain derived metastatic prostate cancer DU-145 cells are effectively inhibited in vitro by guava (Psidium gujava L.) leaf extracts

Kuan Chou Chen, Chiu Lan Hsieh, Chiung Chi Peng, Hsiu Mei Hsieh, Han Sun Chiang, Kuan Dar Huang, Robert Y. Peng

Research output: Contribution to journalArticle

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Abstract

The aqueous extract of Psidium guajava L. (PE) inhibited the cancer cell DU-145 in a dose- and time-dependent manner. At 1.0 mg/mL, PE reduced the viability of PCa DU-145 (the androgen independent PCa cells) to 36.1 and 3.59%, respectively after 48 h and 72 h of incubations. The absolute cell viability suppressing capability (VSC)AC could reach 262.5 cells-mL-h/mg on exposure to PE for 72 h, corresponding to the safe ranges, i.e. the percent viability suppressing rates (PVSR) of 2.72 and 2.41 folds for DU-145 comparing to PZ-HPV-7 cells when treated with PE at 0.5 and 1.0 mg/mL respectively for 72 h. In addition, the colony forming capability of DU-145 cells was apparently lowered. The suppressing rates of which reached 8.09 and 5.96 colony/mg/day for D-145 and PZ-HPV-7 cells, respectively within the concentration range of PE at 0.1∼0.25 mg/mL. Cell cycle arrests at G0/G1 phase in both cells were observed by TUNEL assay and flow cytometric analysis, yet more prominently evident in DU-145. In addition, suppression of the matrix metalloproteinases MMP-2 and MMP-9, and the upregulation of active caspase-3 at 0.10 to 1.0 mg/mL in DU-145 were also effected in a dose-dependent manner by PE at 0.25 to 1.0 mg/mL, implicating a potent anti-metastasis power of PE. Conclusively, we ascribe the anticancer activity of PE to its extraordinarily high polyphenolic (165.61 ± 10.39 mg/g) and flavonoid (82.85 ± 0.22 mg/g) contents. Furthermore, PE might be useful for treatment of brain derived metastatic cancers such as DU-145, acting simultaneously as both a chemopreventive and a chemotherapeutic.

Original languageEnglish
Pages (from-to)93-106
Number of pages14
JournalNutrition and Cancer
Volume58
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

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Psidium
Prostatic Neoplasms
Brain
Matrix Metalloproteinases
Memantine
Cell Cycle Resting Phase
Matrix Metalloproteinase 2
In Situ Nick-End Labeling
G1 Phase
Cell Cycle Checkpoints
In Vitro Techniques
Flavonoids
Caspase 3
Androgens
Neoplasms
Cell Survival
Up-Regulation
Neoplasm Metastasis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Oncology
  • Nutrition and Dietetics
  • Cancer Research

Cite this

Brain derived metastatic prostate cancer DU-145 cells are effectively inhibited in vitro by guava (Psidium gujava L.) leaf extracts. / Chen, Kuan Chou; Hsieh, Chiu Lan; Peng, Chiung Chi; Hsieh, Hsiu Mei; Chiang, Han Sun; Huang, Kuan Dar; Peng, Robert Y.

In: Nutrition and Cancer, Vol. 58, No. 1, 01.01.2007, p. 93-106.

Research output: Contribution to journalArticle

Chen, Kuan Chou ; Hsieh, Chiu Lan ; Peng, Chiung Chi ; Hsieh, Hsiu Mei ; Chiang, Han Sun ; Huang, Kuan Dar ; Peng, Robert Y. / Brain derived metastatic prostate cancer DU-145 cells are effectively inhibited in vitro by guava (Psidium gujava L.) leaf extracts. In: Nutrition and Cancer. 2007 ; Vol. 58, No. 1. pp. 93-106.
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abstract = "The aqueous extract of Psidium guajava L. (PE) inhibited the cancer cell DU-145 in a dose- and time-dependent manner. At 1.0 mg/mL, PE reduced the viability of PCa DU-145 (the androgen independent PCa cells) to 36.1 and 3.59{\%}, respectively after 48 h and 72 h of incubations. The absolute cell viability suppressing capability (VSC)AC could reach 262.5 cells-mL-h/mg on exposure to PE for 72 h, corresponding to the safe ranges, i.e. the percent viability suppressing rates (PVSR) of 2.72 and 2.41 folds for DU-145 comparing to PZ-HPV-7 cells when treated with PE at 0.5 and 1.0 mg/mL respectively for 72 h. In addition, the colony forming capability of DU-145 cells was apparently lowered. The suppressing rates of which reached 8.09 and 5.96 colony/mg/day for D-145 and PZ-HPV-7 cells, respectively within the concentration range of PE at 0.1∼0.25 mg/mL. Cell cycle arrests at G0/G1 phase in both cells were observed by TUNEL assay and flow cytometric analysis, yet more prominently evident in DU-145. In addition, suppression of the matrix metalloproteinases MMP-2 and MMP-9, and the upregulation of active caspase-3 at 0.10 to 1.0 mg/mL in DU-145 were also effected in a dose-dependent manner by PE at 0.25 to 1.0 mg/mL, implicating a potent anti-metastasis power of PE. Conclusively, we ascribe the anticancer activity of PE to its extraordinarily high polyphenolic (165.61 ± 10.39 mg/g) and flavonoid (82.85 ± 0.22 mg/g) contents. Furthermore, PE might be useful for treatment of brain derived metastatic cancers such as DU-145, acting simultaneously as both a chemopreventive and a chemotherapeutic.",
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