Bladder Dysfunction in Rats With Metabolic Syndrome Induced by Long-Term Fructose Feeding

Wei Chia Lee, Chiang Ting Chien, Hong Jeng Yu, Shih Wei Lee

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Purpose: We studied the effects of long-term fructose feeding and metabolic perturbations on bladder function in rats. Materials and Methods: Female Wistar rats were fed a fructose enriched (60%) or control diet for 3 and 6 months. In vitro contractile responses to electrical field stimulation, KCl, carbachol and adenosine triphosphate, and in vivo cystometry were used to evaluate bladder function. Tissue staining and electron microscopy were also performed to evaluate the structural changes in rats subjected to metabolic syndrome. Biochemical and physiological data were compared between fructose fed rats and age matched controls. Results: All rats fed a fructose enriched diet for 3 months showed insulin resistance, hyperinsulinemia, hypertriglyceridemia and hypertension. These fructose fed rats showed a decreased contractile response to high concentrations of KCl but not to other parameters tested compared to controls. Eight of the 12 rats (66.7%) showed abnormal cystometry, mainly by increased phasic contractions. In 6-month fructose fed rats contractile responses to electrical field stimulation, KCl and carbachol were decreased significantly. However, responsiveness to high concentrations of adenosine triphosphate was significantly increased. Morphological studies in fructose fed rats showed swollen mitochondria in bladder smooth muscle, increased leukocyte infiltration between interstitial tissue and neutrophil adhesion around the endothelium of vessels. Conclusions: Our data show that a significant proportion of fructose fed rats show time related alterations in bladder biochemical, morphological and functional properties. The proinflammation and myopathy of the bladder induced by metabolic perturbations have important roles in causing bladder dysfunction.

Original languageEnglish
Pages (from-to)2470-2476
Number of pages7
JournalJournal of Urology
Volume179
Issue number6
DOIs
Publication statusPublished - 2008 Jun 1

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Fructose
Urinary Bladder
Carbachol
Electric Stimulation
Adenosine Triphosphate
Tissue Adhesions
Diet
Hypertriglyceridemia
Hyperinsulinism
Muscular Diseases
Endothelium
Smooth Muscle
Insulin Resistance
Wistar Rats
Electron Microscopy
Mitochondria
Neutrophils
Leukocytes
Staining and Labeling
Hypertension

Keywords

  • Wistar
  • bladder
  • insulin resistance
  • metabolic syndrome X
  • rats
  • urination disorders

ASJC Scopus subject areas

  • Urology

Cite this

Bladder Dysfunction in Rats With Metabolic Syndrome Induced by Long-Term Fructose Feeding. / Lee, Wei Chia; Chien, Chiang Ting; Yu, Hong Jeng; Lee, Shih Wei.

In: Journal of Urology, Vol. 179, No. 6, 01.06.2008, p. 2470-2476.

Research output: Contribution to journalArticle

Lee, Wei Chia ; Chien, Chiang Ting ; Yu, Hong Jeng ; Lee, Shih Wei. / Bladder Dysfunction in Rats With Metabolic Syndrome Induced by Long-Term Fructose Feeding. In: Journal of Urology. 2008 ; Vol. 179, No. 6. pp. 2470-2476.
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