Biofunctionalized magnetic nanoparticles for specifically detecting biomarkers of Alzheimer's disease in vitro

Che Chuan Yang, Shieh-Yueh Yang, Jen Jie Chieh, Herng Er Horng, Chin Yih Hong, Hong-Chang Yang, K. H. Chen, B. Y. Shih, Ta Fu Chen, Ming Jang Chiu

Research output: Contribution to journalArticlepeer-review

73 Citations (Scopus)

Abstract

Magnetic nanoparticles biofunctionalized with antibodies against β-amyloid-40 (Aβ-40) and Aβ-42, which are promising biomarkers related to Alzheimer's disease (AD), were synthesized. We characterized the size distribution, saturated magnetizations, and stability of the magnetic nanoparticles conjugated with anti-Aβ antibody. In combination with immunomagnetic reduction technology, it is demonstrated such biofunctionalized magnetic nanoparticles are able to label Aβs specifically. The ultralow-detection limits of assaying Aβs in vitro using the magnetic nanoparticles via immunomagnetic reduction are determined to a concentration of ~10 ppt (10 pg/mL). Further, immunomagnetic reduction signals of Aβ-40 and Aβ-42 in human plasma from normal samples and AD patients were analyzed, and the results showed a significant difference between these two groups. These results show the feasibility of using magnetic nanoparticles with Aβs as reagents for assaying low-concentration Aβs through immunomagnetic reduction, and also provide a promising new method for early diagnosis of Alzheimer's disease from human blood plasma.

Original languageEnglish
Pages (from-to)500-505
Number of pages6
JournalACS Chemical Neuroscience
Volume2
Issue number9
DOIs
Publication statusPublished - 2011 May 30

Keywords

  • Immunomagnetic reduction
  • Magnetic nanoparticles
  • β-amyloid

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Cognitive Neuroscience
  • Cell Biology

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