Biofunctionalized magnetic nanoparticles for specifically detecting biomarkers of Alzheimer's disease in vitro

Che Chuan Yang, Shieh Yueh Yang, Jen Jie Chieh, Herng Er Horng, Chin Yih Hong, Hong Chang Yang, K. H. Chen, B. Y. Shih, Ta Fu Chen, Ming Jang Chiu

    Research output: Contribution to journalArticlepeer-review

    73 Citations (Scopus)


    Magnetic nanoparticles biofunctionalized with antibodies against β-amyloid-40 (Aβ-40) and Aβ-42, which are promising biomarkers related to Alzheimer's disease (AD), were synthesized. We characterized the size distribution, saturated magnetizations, and stability of the magnetic nanoparticles conjugated with anti-Aβ antibody. In combination with immunomagnetic reduction technology, it is demonstrated such biofunctionalized magnetic nanoparticles are able to label Aβs specifically. The ultralow-detection limits of assaying Aβs in vitro using the magnetic nanoparticles via immunomagnetic reduction are determined to a concentration of ~10 ppt (10 pg/mL). Further, immunomagnetic reduction signals of Aβ-40 and Aβ-42 in human plasma from normal samples and AD patients were analyzed, and the results showed a significant difference between these two groups. These results show the feasibility of using magnetic nanoparticles with Aβs as reagents for assaying low-concentration Aβs through immunomagnetic reduction, and also provide a promising new method for early diagnosis of Alzheimer's disease from human blood plasma.

    Original languageEnglish
    Pages (from-to)500-505
    Number of pages6
    JournalACS Chemical Neuroscience
    Issue number9
    Publication statusPublished - 2011 May 30


    • Immunomagnetic reduction
    • Magnetic nanoparticles
    • β-amyloid

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology
    • Cognitive Neuroscience
    • Cell Biology

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