Biofunctionalized magnetic nanoparticles for in vitro labeling and in vivo locating specific biomolecules

C. C. Wu; L. Y. Lin; L. C. Lin; H. C. Huang; Y. F. Yang; Y. B. Liu; M. C. Tsai; Y. L. Gao; W. C. Wang; S. W. Hung; S. Y. Yang; H. E. Horng; H. C. Yang; W. Y.I. Tseng; H. I. Yeh; C. F. Hsuan; T. L. Lee; W. K. Tseng

doi:10.1063/1.2907486

Biofunctionalized magnetic nanoparticles for in vitro labeling and in vivo locating specific biomolecules

C. C. Wu, L. Y. Lin, L. C. Lin, H. C. Huang, Y. F. Yang, Y. B. Liu, M. C. Tsai, Y. L. Gao, W. C. Wang, S. W. Hung, S. Y. Yang, H. E. Horng, H. C. Yang, W. Y.I. Tseng, H. I. Yeh, C. F. Hsuan, T. L. Lee, W. K. Tseng

Undergraduate Program of Electro-Optical Engineering

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

In this work, we developed processes to biofunctionalize magnetic nanoparticles dispersed in phosphate buffer saline solution. For future clinical utility, magnetic nanoparticles were biofunctionalized with anti-vascular cell adhesion molecule-1 (VCAM-1) to label the VCAM-1 molecule, which served as an indicator for the lesions prone to vulnerable atherosclerotic plaque formation. The biofunctionalized magnetic nanoparticles were used to magnetically label, in vitro, cells expressing VCAM-1, as well as to locate the vulnerable aortic lesions of hypercholesterolemic rabbits with the aid of magnetic resonance imaging. In addition to demonstrating the feasibility of using biofunctionalized magnetic nanoparticles for biomolecule assays, the relevant physical mechanisms are discussed.

Original language	English
Article number	142504
Journal	Applied Physics Letters
Volume	92
Issue number	14
DOIs	https://doi.org/10.1063/1.2907486
Publication status	Published - 2008

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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Wu, C. C., Lin, L. Y., Lin, L. C., Huang, H. C., Yang, Y. F., Liu, Y. B., Tsai, M. C., Gao, Y. L., Wang, W. C., Hung, S. W., Yang, S. Y., Horng, H. E., Yang, H. C., Tseng, W. Y. I., Yeh, H. I., Hsuan, C. F., Lee, T. L., & Tseng, W. K. (2008). Biofunctionalized magnetic nanoparticles for in vitro labeling and in vivo locating specific biomolecules. Applied Physics Letters, 92(14), Article 142504. https://doi.org/10.1063/1.2907486

Wu, CC, Lin, LY, Lin, LC, Huang, HC, Yang, YF, Liu, YB, Tsai, MC, Gao, YL, Wang, WC, Hung, SW, Yang, SY, Horng, HE, Yang, HC, Tseng, WYI, Yeh, HI, Hsuan, CF, Lee, TL & Tseng, WK 2008, 'Biofunctionalized magnetic nanoparticles for in vitro labeling and in vivo locating specific biomolecules', Applied Physics Letters, vol. 92, no. 14, 142504. https://doi.org/10.1063/1.2907486

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title = "Biofunctionalized magnetic nanoparticles for in vitro labeling and in vivo locating specific biomolecules",

abstract = "In this work, we developed processes to biofunctionalize magnetic nanoparticles dispersed in phosphate buffer saline solution. For future clinical utility, magnetic nanoparticles were biofunctionalized with anti-vascular cell adhesion molecule-1 (VCAM-1) to label the VCAM-1 molecule, which served as an indicator for the lesions prone to vulnerable atherosclerotic plaque formation. The biofunctionalized magnetic nanoparticles were used to magnetically label, in vitro, cells expressing VCAM-1, as well as to locate the vulnerable aortic lesions of hypercholesterolemic rabbits with the aid of magnetic resonance imaging. In addition to demonstrating the feasibility of using biofunctionalized magnetic nanoparticles for biomolecule assays, the relevant physical mechanisms are discussed.",

author = "Wu, {C. C.} and Lin, {L. Y.} and Lin, {L. C.} and Huang, {H. C.} and Yang, {Y. F.} and Liu, {Y. B.} and Tsai, {M. C.} and Gao, {Y. L.} and Wang, {W. C.} and Hung, {S. W.} and Yang, {S. Y.} and Horng, {H. E.} and Yang, {H. C.} and Tseng, {W. Y.I.} and Yeh, {H. I.} and Hsuan, {C. F.} and Lee, {T. L.} and Tseng, {W. K.}",

note = "Funding Information: This work is supported by the National Science Council of Taiwan under Grant Nos. 95-2120-M-003-001, 95-2112-M-003-017-MY2, 95-2112-M-003-019, 95-2221-E-212-054-MY3, 95-2752-M-002-016-PAE, and 96-2314-B-002-150-MY3.",

year = "2008",

doi = "10.1063/1.2907486",

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volume = "92",

journal = "Applied Physics Letters",

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T1 - Biofunctionalized magnetic nanoparticles for in vitro labeling and in vivo locating specific biomolecules

AU - Wu, C. C.

AU - Lin, L. Y.

AU - Lin, L. C.

AU - Huang, H. C.

AU - Yang, Y. F.

AU - Liu, Y. B.

AU - Tsai, M. C.

AU - Gao, Y. L.

AU - Wang, W. C.

AU - Hung, S. W.

AU - Yang, S. Y.

AU - Horng, H. E.

AU - Yang, H. C.

AU - Tseng, W. Y.I.

AU - Yeh, H. I.

AU - Hsuan, C. F.

AU - Lee, T. L.

AU - Tseng, W. K.

N1 - Funding Information: This work is supported by the National Science Council of Taiwan under Grant Nos. 95-2120-M-003-001, 95-2112-M-003-017-MY2, 95-2112-M-003-019, 95-2221-E-212-054-MY3, 95-2752-M-002-016-PAE, and 96-2314-B-002-150-MY3.

PY - 2008

Y1 - 2008

N2 - In this work, we developed processes to biofunctionalize magnetic nanoparticles dispersed in phosphate buffer saline solution. For future clinical utility, magnetic nanoparticles were biofunctionalized with anti-vascular cell adhesion molecule-1 (VCAM-1) to label the VCAM-1 molecule, which served as an indicator for the lesions prone to vulnerable atherosclerotic plaque formation. The biofunctionalized magnetic nanoparticles were used to magnetically label, in vitro, cells expressing VCAM-1, as well as to locate the vulnerable aortic lesions of hypercholesterolemic rabbits with the aid of magnetic resonance imaging. In addition to demonstrating the feasibility of using biofunctionalized magnetic nanoparticles for biomolecule assays, the relevant physical mechanisms are discussed.

AB - In this work, we developed processes to biofunctionalize magnetic nanoparticles dispersed in phosphate buffer saline solution. For future clinical utility, magnetic nanoparticles were biofunctionalized with anti-vascular cell adhesion molecule-1 (VCAM-1) to label the VCAM-1 molecule, which served as an indicator for the lesions prone to vulnerable atherosclerotic plaque formation. The biofunctionalized magnetic nanoparticles were used to magnetically label, in vitro, cells expressing VCAM-1, as well as to locate the vulnerable aortic lesions of hypercholesterolemic rabbits with the aid of magnetic resonance imaging. In addition to demonstrating the feasibility of using biofunctionalized magnetic nanoparticles for biomolecule assays, the relevant physical mechanisms are discussed.

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Biofunctionalized magnetic nanoparticles for in vitro labeling and in vivo locating specific biomolecules

Abstract

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